ArticlePloS one2013
Detection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02150031 (Bacteraemia Secondary to Tooth Extraction), which is not on this map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Bacteraemia Secondary to Tooth Extraction: a Randomized Clinical Trial on Efficacy of Three Different Chlorhexidine Prophylaxis Protocols
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Oral hygiene protocols reduce the severity and incidence of oral mucositis during antineoplastic treatment: a systematic review and meta-analysis of randomized and non-randomized clinical trials.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2023Pooled it
- Post-tooth extraction bacteraemia: a randomized clinical trial on the efficacy of chlorhexidine prophylaxis.PloS one · 2015Trial
- Effects of chlorhexidine preprocedural rinse on bacteremia in periodontal patients: a randomized clinical trial.Journal of applied oral science : revista FOBTrial
- The Oral-Gut-Systemic Axis: Emerging Insights into Periodontitis, Microbiota Dysbiosis, and Systemic Disease Interplay.Diagnostics (Basel, Switzerland) · 2025Review
- Mapping Review of the Correlations Between Periodontitis, Dental Caries, and Endocarditis.Dentistry journal · 2025Review
- Investigation of the Blood Microbiome in Horses With Fever of Unknown Origin.Veterinary medicine and science · 2025Article
- Evaluation of Peri-Implantitis Bone Defect Healing: Comparing the Efficacy of Small-Particle Dentin and Bio-Oss in Bone Density Attenuation.Journal of clinical medicine · 2024Article
- How Can Imbalance in Oral Microbiota and Immune Response Lead to Dental Implant Problems?International journal of molecular sciences · 2023Review
- Classical Microbiological Diagnostics of Bacteremia: Are the Negative Results Really Negative? What is the Laboratory Result Telling Us About the "Gold Standard"?Microorganisms · 2020Article
- Comparison of Blood Bacterial Communities in Periodontal Health and Periodontal Disease.Frontiers in cellular and infection microbiology · 2020Article
- Evaluation of Antibiotic Resistance Pattern in Dental Bacteremia Detected by Multiplex PCR Technique.BioMed research international · 2020Article
- Microbiological and Clinical Aspects of CervicofacialDentistry journal · 2019Review
- Analysis of microbial diversity in apple vinegar fermentation process through 16s rDNA sequencing.Food science & nutrition · 2019Article
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- Metagenomic assessment of the interplay between the environment and the genetic diversification of Acinetobacter.Environmental microbiology · 2017Article
- Multiple Approaches Detect the Presence of Fungi in Human Breastmilk Samples from Healthy Mothers.Scientific reports · 2017Article
- Comprehensive detection and identification of bacterial DNA in the blood of patients with sepsis and healthy volunteers using next-generation sequencing method - the observation of DNAemia.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- Comparison of microbial DNA enrichment tools for metagenomic whole genome sequencing.Journal of microbiological methods · 2016Article
- Relationship between Milk Microbiota, Bacterial Load, Macronutrients, and Human Cells during Lactation.Frontiers in microbiology · 2016Article
- Evaluation of a Commercial Multiplex PCR Assay for Detection of Pathogen DNA in Blood from Patients with Suspected Sepsis.PloS one · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe current manuscript aims to determine the prevalence, duration and bacterial diversity of bacteraemia following dental extractions using conventional culture-dependent methods and 16S rDNA pyrosequencing.
methodsThe study group included 8 patients undergoing dental extractions under general anaesthesia. Peripheral venous blood samples were collected at baseline, 30 seconds and 15 minutes after the dental extractions. Blood samples were analysed for bacteraemia applying conventional microbiological cultures under aerobic and anaerobic conditions as well as pyrosequencing using universal bacterial primers that target the 16S ribosomal DNA gene.
resultsTransient bacteremia was detected by culture-based methods in one sample at baseline time, in eight samples at 30 seconds, and in six samples at 15 minutes after surgical procedure; whereas bacteraemia was detected only in five blood samples at 30 seconds after dental extraction by using pyrosequencing. By applying conventional microbiological methods, a single microbial species was detected in six patients, and Streptococcus viridans was the most frequently cultured identified bacterium. By using pyrosequencing approaches however, the estimated blood microbial diversity after dental extractions was 13.4±1.7 bacterial families and 22.8±1.1 genera per sample.
conclusionThe application of 16S rDNA pyrosequencing underestimated the prevalence and duration of bacteraemia following dental extractions, presumably due to not reaching the minimum DNA required for PCR amplification. However, this molecular technique, unlike conventional culture-dependent methods, revealed an extraordinarily high bacterial diversity of post-extraction bacteraemia. We propose that microorganisms recovered by culture may be only the tip of an iceberg of a really diverse microbiota whose viability and potential pathogenicity should be further studied.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.