ArticlePloS one2013
Distinguishing body lice from head lice by multiplex real-time PCR analysis of the Phum_PHUM540560 gene.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Prevalence, Risk Factors, and Phylogenetic Characterization of Pediculus humanus capitis Among Primary Schoolchildren in Algeria.Acta parasitologica · 2026Article
- Impact factors and genetic characteristics of head lice infestation in schoolchildren: a cross-sectional study from 2018 to 2023 in central China.Parasites & vectors · 2025Article
- Retraction: The presence ofFrontiers in cellular and infection microbiology · 2025Article
- Body Louse Pathogen Surveillance among Persons Experiencing Homelessness, Canada, 2020-2021.Emerging infectious diseases · 2024Article
- Retraction: Evidence That Head and Body Lice on Homeless Persons Have the Same Genotype.PloS one · 2024Article
- Correction: Distinguishing Body Lice from Head Lice by Multiplex Real-Time PCR Analysis of the Phum_PHUM540560 Gene.PloS one · 2024Article
- First Detection ofTropical medicine and infectious disease · 2023Article
- Alternative splicing as a source of phenotypic diversity.Nature reviews. Genetics · 2022Review
- Human pediculosis, a global public health problem.Infectious diseases of poverty · 2022Review
- High Genetic Diversity andFrontiers in cellular and infection microbiology · 2022Article
- Expression of Concern: Distinguishing Body Lice from Head Lice by Multiplex Real-Time PCR Analysis of the Phum_PHUM540560 Gene.PloS one · 2022Article
- Molecular Characterization and Genetic Diversity of Haplogroup E Human Lice in Guinea, West Africa.Microorganisms · 2021Article
- Where Are We With Human Lice? A Review of the Current State of Knowledge.Frontiers in cellular and infection microbiology · 2019Review
- Detection of bacterial pathogens in clade E head lice collected from Niger's refugees in Algeria.Parasites & vectors · 2018Article
- RETRACTED: Body lice of homeless people reveal the presence of several emerging bacterial pathogens in northern Algeria.PLoS neglected tropical diseases · 2018Article
- Bartonella quintana and Typhus Group Rickettsiae Exposure among Homeless Persons, Bogotá, Colombia.Emerging infectious diseases · 2017Article
- Genetic diversity of the human head lice, Pediculus humanus capitis, among primary school girls in Saudi Arabia, with reference to their prevalence.Parasitology research · 2017Article
- Detection of bacterial pathogens including potential new species in human head lice from Mali.PloS one · 2017Article
- Management and Treatment of Human Lice.BioMed research international · 2016Review
- A New Clade of African Body and Head Lice Infected by Bartonella quintana and Yersinia pestis-Democratic Republic of the Congo.The American journal of tropical medicine and hygiene · 2015Article
Corrections and comments
- Correction · 2024-10-01Concerns/Issues about Data · Error in Text · Investigation by Company/Institution · Investigation by Journal/Publisher · Upgrade/Update of Prior Notice(s) ·
- Expression of concern · 2022-12-13Investigation by Journal/Publisher · Updated to Correction ·
- Erratum issued
- Expression of concern
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBody louse or head louse? Once removed from their environment, body and head lice are indistinguishable. Neither the morphological criteria used since the mid-18th century nor the various genetic studies conducted since the advent of molecular biology tools have allowed body lice and head lice to be differentiated. In this work, using a portion of the Phum_PHUM540560 gene from the body louse, we aimed to develop a multiplex real-time polymerase chain reaction (PCR) assay to differentiate between body and head lice in a single reaction. MATERIALS AND
methodsA total of 142 human lice were collected from mono-infested hosts from 13 countries on five continents. We first identified the louse clade using a cytochrome b (CYTB) PCR sequence alignment. We then aligned a fragment of the Phum_PHUM540560 gene amplified from head and body lice to design-specific TaqMan(©) FAM- and VIC-labeled probes.
resultsAll the analyzed lice were Clade A lice. A total of 22 polymorphisms between the body and head lice were characterized. The multiplex real-time PCR analysis enabled the body and head lice to be distinguished in two hours. This method is simple, with 100% specificity and sensitivity.
conclusionsWe confirmed that the Phum_PHUM540560 gene is a useful genetic marker for the study of lice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.