ArticlePloS one2013
Validation of reference genes in Solenopsis invicta in different developmental stages, castes and tissues.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed.
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- Family before work: task reversion in workers of the red imported fire ant, Solenopsis invicta in the presence of brood.Scientific reports · 2023Article
- Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR inFrontiers in physiology · 2023Article
- Identification and Validation of Reference Genes for Expression Analysis in Nitrogen-Fixing Bacteria under Environmental Stress.Life (Basel, Switzerland) · 2022Article
- Selection and validation of reference genes for RT-qPCR-based analyses ofFrontiers in physiology · 2022Article
- Validation of Potential Reference Genes for Real-Time qPCR Analysis in Pharaoh Ant,Frontiers in physiology · 2022Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Normalization inFrontiers in physiology · 2022Article
- Comparative RNA-Seq Analyses ofGenes · 2021Article
- Comparison of gene expression in the red imported fire ant (Solenopsis invicta) under different temperature conditions.Scientific reports · 2021Article
- Selection of reference genes for gene expression analysis in Liriodendron hybrids' somatic embryogenesis and germinative tissues.Scientific reports · 2021Article
- Reference Gene Selection for Normalizing Gene Expression inFrontiers in physiology · 2021Article
- Identification and Evaluation of Suitable Reference Genes for RT-qPCR Analysis inFrontiers in physiology · 2021Article
- Reference gene selection for quantitative real-time PCR (qRT-PCR) expression analysis in Galium aparine L.PloS one · 2020Article
- Selection of reference genes for tissue/organ samples of adults of Eucryptorrhynchus scrobiculatus.PloS one · 2020Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
To accurately assess gene expression levels, it is essential to normalize real-time quantitative PCR (RT-qPCR) data with suitable internal reference genes. For the red imported fire ant, Solenopsis invicta, reliable reference genes to assess the transcript expression levels of the target genes have not been previously investigated. In this study, we examined the expression levels of five candidate reference genes (rpl18, ef1-beta, act, GAPDH, and tbp) in different developmental stages, castes and tissues of S. invicta. To evaluate the suitability of these genes as endogenous controls, three software-based approaches (geNorm, BestKeeper and NormFinder) and one web-based comprehensive tool (RefFinder) were used to analyze and rank the tested genes. Furthermore, the optimal number of reference gene(s) was determined by the pairwise variation value. Our data showed that two of the five candidate genes, rpl18 and ef1-beta, were the most suitable reference genes because they have the most stable expression among different developmental stages, castes and tissues in S. invicta. Although widely used as reference gene in other species, in S. invicta the act gene has high variation in expression and was consequently excluded as a reliable reference gene. The two validated reference genes, rpl18 and ef1-beta, can be widely used for quantification of target gene expression with RT-qPCR technology in S. invicta.
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