ArticlePloS one2013
Improving ethanol tolerance of Escherichia coli by rewiring its global regulator cAMP receptor protein (CRP).
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 88 citations in OpenAlex.
- A self-iterative orthogonal base-editing platform enables multiplex N-to-N diversification and genome-scale functional screening in Escherichia coli.Nucleic acids research · 2026Article
- Klebsiella pneumoniae under xylose pressure: the growth adaptation, antimicrobial susceptibility, global proteomics analysis and role of XylA and XylB proteins.BMC microbiology · 2025Article
- Biological characterization of lipoic acid- and heme-dependent Escherichia coli small colony variants isolated from sheep in Xinjiang, China.Veterinary research communications · 2024Article
- CRISPR-Cas technology secures sustainability through its applications: a review in green biotechnology.3 Biotech · 2023Review
- Production of cellulosic ethanol and value-added products from corn fiber.Bioresources and bioprocessing · 2022Review
- Engineering regulatory networks for complex phenotypes in E. coli.Nature communications · 2020Article
- Improved acid-stress tolerance of Lactococcus lactis NZ9000 and Escherichia coli BL21 by overexpression of the anti-acid component recT.Journal of industrial microbiology & biotechnology · 2018Article
- Understanding and engineering alcohol-tolerant bacteria using OMICS technology.World journal of microbiology & biotechnology · 2018Review
- Identification and manipulation of a novel locus to improve cell tolerance to short-chain alcohols in Escherichia coli.Journal of industrial microbiology & biotechnology · 2018Article
- Improved xylose tolerance and 2,3-butanediol production ofBiotechnology for biofuels · 2018Article
- Systems-level understanding of ethanol-induced stresses and adaptation in E. coli.Scientific reports · 2017Article
- ImprovingBiotechnology for biofuels · 2017Article
- Using global transcription machinery engineering (gTME) to improve ethanol tolerance of Zymomonas mobilis.Microbial cell factories · 2016Article
- Toxicological challenges to microbial bioethanol production and strategies for improved tolerance.Ecotoxicology (London, England) · 2015Review
- Folding Optimization In Vivo Uncovers New Chaperones.Journal of molecular biology · 2015Article
- A transcriptional regulator Sll0794 regulates tolerance to biofuel ethanol in photosynthetic Synechocystis sp. PCC 6803.Molecular & cellular proteomics : MCP · 2014Article
- Correcting direct effects of ethanol on translation and transcription machinery confers ethanol tolerance in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Enhancing stress-resistance for efficient microbial biotransformations by synthetic biology.Frontiers in bioengineering and biotechnology · 2014Review
- Improving acetate tolerance of Escherichia coli by rewiring its global regulator cAMP receptor protein (CRP).PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major challenge in bioethanol fermentation is the low tolerance of the microbial host towards the end product bioethanol. Here we report to improve the ethanol tolerance of E. coli from the transcriptional level by engineering its global transcription factor cAMP receptor protein (CRP), which is known to regulate over 400 genes in E. coli. Three ethanol tolerant CRP mutants (E1- E3) were identified from error-prone PCR libraries. The best ethanol-tolerant strain E2 (M59T) had the growth rate of 0.08 h(-1) in 62 g/L ethanol, higher than that of the control at 0.06 h(-1). The M59T mutation was then integrated into the genome to create variant iE2. When exposed to 150 g/l ethanol, the survival of iE2 after 15 min was about 12%, while that of BW25113 was <0.01%. Quantitative real-time reverse transcription PCR analysis (RT-PCR) on 444 CRP-regulated genes using OpenArray® technology revealed that 203 genes were differentially expressed in iE2 in the absence of ethanol, whereas 92 displayed differential expression when facing ethanol stress. These genes belong to various functional groups, including central intermediary metabolism (aceE, acnA, sdhD, sucA), iron ion transport (entH, entD, fecA, fecB), and general stress response (osmY, rpoS). Six up-regulated and twelve down-regulated common genes were found in both iE2 and E2 under ethanol stress, whereas over one hundred common genes showed differential expression in the absence of ethanol. Based on the RT-PCR results, entA, marA or bhsA was knocked out in iE2 and the resulting strains became more sensitive towards ethanol.
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