ArticlePloS one2013
Pathogenic role of basic calcium phosphate crystals in destructive arthropathies.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 101 citations in OpenAlex.
- Characterization of calcifications in posterior horn of human meniscus using micro-computed tomography.Osteoarthritis and cartilage open · 2026Article
- Voxel-wise deep learning segmentation of hydroxyapatite and iodine in spectral photon-counting CT: A quantitative phantom study.PloS one · 2026Article
- Pyroptosis: A spoiler of peaceful coexistence between cells in degenerative bone and joint diseases.Journal of advanced research · 2025Review
- A new ex vivo human model of osteoarthritis cartilage calcification.Rheumatology (Oxford, England) · 2025Article
- The translational value of calcium pyrophosphate deposition disease experimental mouse models.Frontiers in medicine · 2024Review
- Synovial Fluid from Patients with Osteoarthritis Shows Different Inflammatory Features Depending on the Presence of Calcium Pyrophosphate Crystals.International journal of molecular sciences · 2023Article
- Crystal arthropathies and osteoarthritis-where is the link?Skeletal radiology · 2023Review
- Targeting inflammasome-dependent mechanisms as an emerging pharmacological approach for osteoarthritis therapy.iScience · 2022Review
- Inflammasomes and the IL-1 Family in Bone Homeostasis and Disease.Current osteoporosis reports · 2022Review
- IκB-ζ signaling promotes chondrocyte inflammatory phenotype, senescence, and erosive joint pathology.Bone research · 2022Article
- Activation of NLRP3 Inflammasome Complexes by Beta-Tricalcium Phosphate Particles and Stimulation of Immune Cell Migration in vivo.Journal of innate immunity · 2022Article
- The Gasotransmitter Hydrogen Sulfide (HAntioxidants (Basel, Switzerland) · 2021Article
- Features and Outcomes of Microcrystalline Arthritis Treated by Biologics: A Retrospective Study.Rheumatology and therapy · 2021Article
- Hyaluronic acid-alendronate conjugate: A macromolecular drug delivery system for intra-articular treatment of osteoarthritis.Osteoarthritis and cartilage open · 2021Article
- Review
- Mitochondria in Injury, Inflammation and Disease of Articular Skeletal Joints.Frontiers in immunology · 2021Review
- Chondrocytes From Osteoarthritic and Chondrocalcinosis Cartilage Represent Different Phenotypes.Frontiers in cell and developmental biology · 2021Article
- The role of the gasotransmitter hydrogen sulfide in pathological calcification.British journal of pharmacology · 2020Review
- Purinergic System Signaling in Metainflammation-Associated Osteoarthritis.Frontiers in medicine · 2020Review
- Urate and osteoarthritis: Evidence for a reciprocal relationship.Joint bone spine · 2019Review
Corrections and comments
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Authors and funding
15 authors at 7 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundbasic calcium phosphate (BCP) crystals are commonly found in osteoarthritis (OA) and are associated with cartilage destruction. BCP crystals induce in vitro catabolic responses with the production of metalloproteases and inflammatory cytokines such as interleukin-1 (IL-1). In vivo, IL-1 production induced by BCP crystals is both dependant and independent of NLRP3 inflammasome. We aimed to clarify 1/ the role of BCP crystals in cartilage destruction and 2/ the role of IL-1 and NLRP3 inflammasome in cartilage degradation related to BCP crystals. METHODOLOGY PRINCIPAL
findingssynovial membranes isolated from OA knees were analysed by alizarin Red and FTIR. Pyrogen free BCP crystals were injected into right knees of WT, NLRP3 -/-, ASC -/-, IL-1α -/- and IL-1β-/- mice and PBS was injected into left knees. To assess the role of IL-1, WT mice were treated by intra-peritoneal injections of anakinra, the IL-1Ra recombinant protein, or PBS. Articular destruction was studied at d4, d17 and d30 assessing synovial inflammation, proteoglycan loss and chondrocyte apoptosis. BCP crystals were frequently found in OA synovial membranes including low grade OA. BCP crystals injected into murine knee joints provoked synovial inflammation characterized by synovial macrophage infiltration that persisted at day 30, cartilage degradation as evidenced by loss of proteoglycan staining by Safranin-O and concomitant expression of VDIPEN epitopes, and increased chondrocyte apoptosis. BCP crystal-induced synovitis was totally independent of IL-1α and IL-1β signalling and no alterations of inflammation were observed in mice deficient for components of the NLRP3-inflammasome, IL-1α or IL-1β. Similarly, treatment with anakinra did not prevent BCP crystal effects. In vitro, BCP crystals elicited enhanced transcription of matrix degrading and pro-inflammatory genes in macrophages. CONCLUSIONS SIGNIFICANCE: intra-articular BCP crystals can elicit synovial inflammation and cartilage degradation suggesting that BCP crystals have a direct pathogenic role in OA. The effects are independent of IL-1 and NLRP3 inflammasome.
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