ArticlePloS one2013
investigating acid production by Streptococcus mutans with a surface-displayed pH-sensitive green fluorescent protein.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
35 citing papers in PubMed, 54 citations in OpenAlex.
- Unraveling Oral Dysbiosis: Microbial Complexity in Common Oral Diseases.MicrobiologyOpen · 2026Review
- Design, development, and validation of new fluorescent strains for studying oral streptococci.Microbiology spectrum · 2025Article
- Promising potential effects of resveratrol on oral and dental health maintenance: a comprehensive review.Naunyn-Schmiedeberg's archives of pharmacology · 2025Review
- Real-time acid production and extracellular matrix formation in mature biofilms of threeBiofilm · 2024Article
- The Mechanisms and Application Value of Postbiotics in Caries Prevention and Management.Oral health & preventive dentistry · 2024Review
- Pine-Oil-Derived Sodium Resinate Inhibits Growth and Acid Production ofDentistry journal · 2024Article
- The Antimicrobial Potential of the Hop (Pharmaceuticals (Basel, Switzerland) · 2024Article
- Oral Microbiome and Alzheimer's Disease.Microorganisms · 2023Article
- Effects of DJK-5 and chlorhexidine on exopolysaccharide volume and pH in oral biofilms.BMC oral health · 2023Article
- Stoichiometric models of sucrose and glucose fermentation by oral streptococci: Implications for free acid formation and enamel demineralization.Dental materials : official publication of the Academy of Dental Materials · 2023Article
- Anti-Bacterial Effect of Cannabidiol against the CariogenicInternational journal of molecular sciences · 2022Article
- A study on Sr/Zn phytate complexes: structural properties and antimicrobial synergistic effects against Streptococcus mutans.Scientific reports · 2022Article
- Usnic acid deteriorates acidogenicity, acidurance and glucose metabolism of Streptococcus mutans through downregulation of two-component signal transduction systems.Scientific reports · 2021Article
- The Relationship Between Functional Dyspepsia, PPI Therapy, and the Gastric Microbiome.Kansas journal of medicine · 2021Review
- Molecular Fingerprint Imaging to Identify Dental Caries Using Raman Spectroscopy.Materials (Basel, Switzerland) · 2020Article
- Modulation of Lipoteichoic Acids and Exopolysaccharides PreventsMolecules (Basel, Switzerland) · 2020Article
- Spatial Correlations and Distribution of Competence Gene Expression in Biofilms ofFrontiers in microbiology · 2020Article
- The Structure of Dental Plaque Microbial Communities in the Transition from Health to Dental Caries and Periodontal Disease.Journal of molecular biology · 2019Review
- Nanoparticles for Oral Biofilm Treatments.ACS nano · 2019Review
- A Chemical Approach to Optimizing Bioactive Glass Dental Composites.Journal of dental research · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Acidogenicity and aciduricity are the main virulence factors of the cavity-causing bacterium Streptococcus mutans. Monitoring at the individual cell level the temporal and spatial distribution of acid produced by this important oral pathogen is central for our understanding of these key virulence factors especially when S. mutans resides in multi-species microbial communities. In this study, we explored the application of pH-sensitive green fluorescent proteins (pHluorins) to investigate these important features. Ecliptic pHluorin was functionally displayed on the cell surface of S. mutans as a fusion protein with SpaP. The resulting strain (O87) was used to monitor temporal and spatial pH changes in the microenvironment of S. mutans cells under both planktonic and biofilm conditions. Using strain O87, we revealed a rapid pH drop in the microenviroment of S. mutans microcolonies prior to the decrease in the macro-environment pH following sucrose fermentation. Meanwhile, a non-uniform pH distribution was observed within S. mutans biofilms, reflecting differences in microbial metabolic activity. Furthermore, strain O87 was successfully used to monitor the S. mutans acid production profiles within dual- and multispecies oral biofilms. Based on these findings, the ecliptic pHluorin allows us to investigate in vivo and in situ acid production and distribution by the cariogenic species S. mutans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.