ArticleJournal of virology2013
Structural features of a picornavirus polymerase involved in the polyadenylation of viral RNA.
Article in Journal of virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Genetic mechanisms underlying the structural elaboration and dissemination of viral internal ribosomal entry sites.Nucleic acids research · 2026Article
- Zoological transmission of encephalomyocarditis virus in the United States: Virus evolution, host ecology, and capsid antigenicity derived from an outbreak.PLoS pathogens · 2026Article
- Binding of Glycyl-tRNA synthetase to Mengovirus RNA stimulates translation.Nucleic acids research · 2026Article
- Enterovirus C recombination groups: RNA sequence similarity and the viral polymerase underpin sexual replication mechanisms.Journal of virology · 2025Article
- Heat shock protein A1 inhibits the replication of foot-and-mouth disease virus by degrading viral RNA polymerase 3D through chaperone-mediated autophagy.Journal of virology · 2025Article
- Multiple functions of the nonstructural protein 3D in picornavirus infection.Frontiers in immunology · 2024Review
- A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection.Cell reports · 2021Article
- E2 ubiquitin-conjugating enzyme UBE2L6 promotes Senecavirus A proliferation by stabilizing the viral RNA polymerase.PLoS pathogens · 2020Article
- An Extended Primer Grip of Picornavirus Polymerase Facilitates Sexual RNA Replication Mechanisms.Journal of virology · 2020Article
- A nucleobase-binding pocket in a viral RNA-dependent RNA polymerase contributes to elongation complex stability.Nucleic acids research · 2020Article
- Article
- Picornavirus RNA Recombination Counteracts Error Catastrophe.Journal of virology · 2019Article
- Propensity of a picornavirus polymerase to slip on potyvirus-derived transcriptional slippage sites.The Journal of general virology · 2019Article
- Picornaviral polymerase structure, function, and fidelity modulation.Virus research · 2017Review
- Poliovirus Polymerase Leu420 Facilitates RNA Recombination and Ribavirin Resistance.Journal of virology · 2016Article
- Mutational analysis of the Potyviridae transcriptional slippage site utilized for expression of the P3N-PIPO and P1N-PISPO proteins.Nucleic acids research · 2016Article
- Characterization of the Role of Hexamer AGUAAA and Poly(A) Tail in Coronavirus Polyadenylation.PloS one · 2016Article
- Picornavirus RNA polyadenylation by 3D(pol), the viral RNA-dependent RNA polymerase.Virus research · 2015Review
- Initiation of protein-primed picornavirus RNA synthesis.Virus research · 2015Review
- Transcriptional slippage in the positive-sense RNA virus family Potyviridae.EMBO reports · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Picornaviruses have 3' polyadenylated RNA genomes, but the mechanisms by which these genomes are polyadenylated during viral replication remain obscure. Based on prior studies, we proposed a model wherein the poliovirus RNA-dependent RNA polymerase (3D(pol)) uses a reiterative transcription mechanism while replicating the poly(A) and poly(U) portions of viral RNA templates. To further test this model, we examined whether mutations in 3D(pol) influenced the polyadenylation of virion RNA. We identified nine alanine substitution mutations in 3D(pol) that resulted in shorter or longer 3' poly(A) tails in virion RNA. These mutations could disrupt structural features of 3D(pol) required for the recruitment of a cellular poly(A) polymerase; however, the structural orientation of these residues suggests a direct role of 3D(pol) in the polyadenylation of RNA genomes. Reaction mixtures containing purified 3D(pol) and a template RNA with a defined poly(U) sequence provided data consistent with a template-dependent reiterative transcription mechanism for polyadenylation. The phylogenetically conserved structural features of 3D(pol) involved in the polyadenylation of virion RNA include a thumb domain alpha helix that is positioned in the minor groove of the double-stranded RNA product and lysine and arginine residues that interact with the phosphates of both the RNA template and product strands.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.