ArticleGenes & development2013
Intronic sequence elements impede exon ligation and trigger a discard pathway that yields functional telomerase RNA in fission yeast.
Article in Genes & development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Article
- Splicing of branchpoint-distant exons is promoted by Cactin, Tls1 and the ubiquitin-fold-activated Sde2.Nucleic acids research · 2022Article
- A putative cap binding protein and the methyl phosphate capping enzyme Bin3/MePCE function in telomerase biogenesis.Nature communications · 2022Article
- Termination of pre-mRNA splicing requires that the ATPase and RNA unwindase Prp43p acts on the catalytic snRNA U6.Genes & development · 2019Article
- Rapidly evolving protointrons in Saccharomyces genomes revealed by a hungry spliceosome.PLoS genetics · 2019Article
- Early splicing functions of fission yeast Prp16 and its unexpected requirement for gene Silencing is governed by intronic features.RNA biology · 2019Article
- Intron specificity in pre-mRNA splicing.Current genetics · 2018Review
- Article
- New perspectives on telomerase RNA structure and function.Wiley interdisciplinary reviews. RNA · 2018Review
- Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast.Nature communications · 2018Article
- Large-scale analysis of branchpoint usage across species and cell lines.Genome research · 2017Article
- Article
- Prevalent and distinct spliceosomal 3'-end processing mechanisms for fungal telomerase RNA.Nature communications · 2015Article
- Diverse mechanisms for spliceosome-mediated 3' end processing of telomerase RNA.Nature communications · 2015Article
- Specific features of telomerase RNA from Hansenula polymorpha.RNA (New York, N.Y.) · 2013Article
- Spliceosome-mediated decay (SMD) regulates expression of nonintronic genes in budding yeast.Genes & development · 2013Article
- Splicing fidelity: DEAD/H-box ATPases as molecular clocks.RNA biology · 2013Review
- Article
- Non-mRNA 3' end formation: how the other half lives.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
Abstract
The fission yeast telomerase RNA (TER1) precursor harbors an intron immediately downstream from its mature 3' end. Unlike most introns, which are removed from precursor RNAs by the spliceosome in two sequential but tightly coupled transesterification reactions, TER1 only undergoes the first cleavage reaction during telomerase RNA maturation. The mechanism underlying spliceosome-mediated 3' end processing has remained unclear. We now demonstrate that a strong branch site (BS), a long distance to the 3' splice site (3' SS), and a weak polypyrimidine (Py) tract act synergistically to attenuate the transition from the first to the second step of splicing. The observation that a strong BS antagonizes the second step of splicing in the context of TER1 suggests that the BS-U2 snRNA interaction is disrupted after the first step and thus much earlier than previously thought. The slow transition from first to second step triggers the Prp22 DExD/H-box helicase-dependent rejection of the cleaved products and Prp43-dependent "discard" of the splicing intermediates. Our findings explain how the spliceosome can function in 3' end processing and provide new insights into the mechanism of splicing.
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