ArticleAnnals of neurology2013
RNA in blood is altered prior to hemorrhagic transformation in ischemic stroke.
Article in Annals of neurology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
39 citing papers in PubMed, 67 citations in OpenAlex.
- A roadmap towards clinical use of molecular biomarkers in acute ischaemic stroke.Nature reviews. Neurology · 2026Review
- Molecular heterogeneity in human stroke - What can we learn from the peripheral blood transcriptome?Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026Review
- Blood RNA Signatures Enable Accurate Discrimination of Stroke Subtype and Onset Time at Hospital Admission.Research square · 2026Article
- Development and Validation of Multiple Machine Learning Models Integrating Neutrophil-Lymphocyte Ratio for Prediction of Hemorrhagic Transformation After Intravenous Thrombolysis in Acute Ischemic Stroke.CNS neuroscience & therapeutics · 2025Article
- Differential Transcript Expression and Alternative RNA Splicing Patterns to Differentiate Focal vs. Generalized-Onset Seizures.Molecular neurobiology · 2025Article
- Retrospective discrimination of PNES and epileptic seizure types using blood RNA signatures.Journal of neurology · 2025Article
- Explainable machine learning model for predicting the outcome of acute ischemic stroke after intravenous thrombolysis.Frontiers in neurology · 2025Article
- Early peripheral blood gene expression associated with good and poor 90-day ischemic stroke outcomes.Journal of neuroinflammation · 2023Article
- A modified mouse model of haemorrhagic transformation associated with tPA administration after thromboembolic stroke.Heliyon · 2023Article
- Hemorrhagic Transformation After Tissue Plasminogen Activator Treatment in Acute Ischemic Stroke.Cellular and molecular neurobiology · 2022Review
- Hemorrhagic Transformation in Acute Ischemic Stroke: A Quantitative Systematic Review.Journal of clinical medicine · 2022Article
- Blood and brain transcriptome analysis reveals APOE genotype-mediated and immune-related pathways involved in Alzheimer disease.Alzheimer's research & therapy · 2022Article
- Bioinformatic analysis and clinical diagnostic value of hsa_circ_0004099 in acute ischemic stroke.PloS one · 2022Article
- Radiomics-based infarct features on CT predict hemorrhagic transformation in patients with acute ischemic stroke.Frontiers in neuroscience · 2022Article
- Protocol for LASER: A Randomized Evaluation and an Associated Registry of Early Anticoagulation With Edoxaban After Ischemic Stroke in Patients With Atrial Fibrillation.Frontiers in neurology · 2021Article
- Whole Blood Transcriptome Analysis in Children with Sickle Cell Anemia.Frontiers in genetics · 2021Article
- Hemorrhagic Transformation After Ischemic Stroke: Mechanisms and Management.Frontiers in neurology · 2021Review
- Hemorrhagic Transformation in Ischemic Stroke and the Role of Inflammation.Frontiers in neurology · 2021Review
- Genetic variation contributes to gene expression response in ischemic stroke: an eQTL study.Annals of clinical and translational neurology · 2020Article
- High Neutrophil-to-Lymphocyte Ratio Predicts Hemorrhagic Transformation in Acute Ischemic Stroke Patients Treated with Intravenous Thrombolysis.International journal of hypertension · 2020Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
Abstract
objectiveHemorrhagic transformation (HT) is a major complication of ischemic stroke that worsens outcomes and increases mortality. Disruption of the blood-brain barrier is a central feature of HT pathogenesis, and leukocytes may contribute to this process. We sought to determine whether ischemic strokes that develop HT have differences in RNA expression in blood within 3 hours of stroke onset prior to treatment with thrombolytic therapy.
methodsStroke patient blood samples were obtained prior to treatment with thrombolysis, and leukocyte RNA was assessed by microarray analysis. Strokes that developed HT (n = 11) were compared to strokes without HT (n = 33) and controls (n = 14). Genes were identified (corrected p < 0.05, fold change ≥|1.2|), and functional analysis was performed. RNA prediction of HT in stroke was evaluated using cross-validation, and in a second stroke cohort (n = 52).
resultsIschemic strokes that developed HT had differential expression of 29 genes in circulating leukocytes prior to treatment with thrombolytic therapy. A panel of 6 genes could predict strokes that later developed HT with 80% sensitivity and 70.2% specificity. Key pathways involved in HT of human stroke are described, including amphiregulin, a growth factor that regulates matrix metalloproteinase-9; a shift in transforming growth factor-β signaling involving SMAD4, INPP5D, and IRAK3; and a disruption of coagulation factors V and VIII.
interpretationIdentified genes correspond to differences in inflammation and coagulation that may predispose to HT in ischemic stroke. Given the adverse impact of HT on stroke outcomes, further evaluation of the identified genes and pathways is warranted to determine their potential as therapeutic targets to reduce HT and as markers of HT risk.
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Registered trials
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