ArticleJournal of lipid research2013
Bile salt-stimulated phospholipid efflux mediated by ABCB4 localized in nonraft membranes.
Article in Journal of lipid research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Bile Acid Detergency as Determinant of Liver Pathology in a Humanized Mouse Model of Progressive Familial Intrahepatic Cholestasis Type 3.Cellular and molecular gastroenterology and hepatology · 2026Article
- Phospholipid biomarkers of coronary heart disease.Journal of pharmaceutical health care and sciences · 2024Review
- The correlation between urinary iodine levels and gallstone risk: elevated iodine intake linked to gallstone occurrence.Frontiers in nutrition · 2024Article
- Effects of Biliary Phospholipids on Cholesterol Crystallization and Growth in Gallstone Formation.Advances in therapy · 2023Review
- In Vivo and In Vitro Models to Study Liver Fibrosis: Mechanisms and Limitations.Cellular and molecular gastroenterology and hepatology · 2023Review
- Article
- RAB10 Interacts with ABCB4 and Regulates Its Intracellular Traffic.International journal of molecular sciences · 2021Article
- A sensitive S-Trap-based approach to the analysis of T cell lipid raft proteome.Journal of lipid research · 2020Article
- Protocols for Enzymatic Fluorometric Assays to Quantify Phospholipid Classes.International journal of molecular sciences · 2020Review
- Structure of the human lipid exporter ABCB4 in a lipid environment.Nature structural & molecular biology · 2020Article
- Cholesterol attenuates cytoprotective effects of phosphatidylcholine against bile salts.Scientific reports · 2017Article
- Thyroid dysfunction, either hyper or hypothyroidism, promotes gallstone formation by different mechanisms.Journal of Zhejiang University. Science. B · 2016Article
- Experimental models of liver fibrosis.Archives of toxicology · 2016Review
- Deficiency of multidrug resistance 2 contributes to cell transformation through oxidative stress.Carcinogenesis · 2016Article
- ABCB4 exports phosphatidylcholine in a sphingomyelin-dependent manner.Journal of lipid research · 2015Article
- Molecular mechanisms for biliary phospholipid and drug efflux mediated by ABCB4 and bile salts.BioMed research international · 2014Review
- Functional characterisation of the maturation of the blood-brain barrier in larval zebrafish.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ABCB4 is necessary for the secretion of phospholipids from hepatocytes into bile and for the protection of cell membranes against bile salts. Lipid rafts are plasma membrane microdomains containing high contents of cholesterol and sphingolipids, which are separated by Triton X-100 extraction or OptiPrep gradient centrifugation. In this study, we investigated the relationship between the function of ABCB4 and lipid rafts using mouse canalicular membranes and HEK293 cells stably expressing ABCB4. ABCB4 and ABCB1 were mainly distributed in nonraft membranes. The expression of ABCB4, but not ABCB1, led to significant increases in the phosphatidylcholine (PC), phosphatidylethanolamine (PE), and sphingomyelin (SM) contents in nonraft membranes and further enrichment of SM and cholesterol in raft membranes. The ABCB4-mediated efflux of PC, PE, and SM was significantly stimulated by taurocholate, while the efflux of PE and SM was much less than that of PC. This ABCB4-mediated efflux was completely abolished by BODIPY-verapamil, which hardly partitioned into raft membranes. In addition, ABCB1 and ABCB4 mediated the efflux of rhodamine 123 and rhodamine 6G from nonraft membranes, which was not affected by taurocholate. We conclude that ABCB4 located in nonrafts, but not in rafts, is predominantly involved in the efflux of phospholipids and other substrates.
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