ArticleClinical and vaccine immunology : CVI2013
Development of immunochromatography-based methods for detection of leptospiral lipopolysaccharide antigen in urine.
Article in Clinical and vaccine immunology : CVI, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.
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Who cites it
18 citing papers in PubMed, 2 syntheses or guidelines pooled it, 48 citations in OpenAlex.
- Accuracy of rapid lateral flow immunoassays for human leptospirosis diagnosis: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2024Pooled it
- Nucleic acid and antigen detection tests for leptospirosis.The Cochrane database of systematic reviews · 2019Pooled it
- Molecular detection and genetic characterisation of a large flood-borne outbreak of human leptospirosis in Jakarta, Indonesia: A retrospective analysis of surveillance data.PLoS neglected tropical diseases · 2026Article
- Early diagnosis and treatment of leptospirosis: Optimizing clinical outcomes.The Journal of infection · 2026Review
- Laboratory Diagnosis of Leptospirosis.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Development of leptospiral virulence-modifying protein detection assay: implications for pathogenesis and diagnostic test development.Microbiology spectrum · 2025Article
- Performance of electrochemical aptasensor as antigen test in clinical samples for early diagnosis of leptospirosis.Scientific reports · 2025Article
- Humoral immune response and changes in peritoneal cell populations in rats immunized against two Leptospira serovars; serovar patoc and serovar pyrogenes.BMC immunology · 2023Article
- A clinical pilot study for the detection of sphingomyelinase in leptospirosis patient's urine at tertiary care hospital.Heliyon · 2023Article
- Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography.Memorias do Instituto Oswaldo Cruz · 2023Article
- Evaluating immunochromatographic test kits for diagnosis of acute human leptospirosis: A systematic review.Heliyon · 2022Review
- A Novel, Inexpensive In-House Immunochromatographic Strip Test for Cryptococcosis Based on the Cryptococcal Glucuronoxylomannan Specific Monoclonal Antibody 18B7.Diagnostics (Basel, Switzerland) · 2021Article
- Leptospiral 3-hydroxyacyl-CoA dehydrogenase as an early urinary biomarker of leptospirosis.Heliyon · 2018Article
- Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis.Scientific reports · 2017Article
- Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species.BMC microbiology · 2015Article
- Lipopolysaccharide Specific Immunochromatography Based Lateral Flow Assay for Serogroup Specific Diagnosis of Leptospirosis in India.PloS one · 2015Article
- Identification of leptospiral 3-hydroxyacyl-CoA dehydrogenase released in the urine of infected hamsters.BMC microbiology · 2014Article
- Authors' response.The Indian journal of medical research · 2013Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptospirosis is an infectious disease caused by the spirochete bacteria Leptospira spp. and is commonly found throughout the world. Diagnosis of leptospirosis performed by culture and microscopic agglutination tests is laborious and time-consuming. Therefore, we aimed to develop a novel immunochromatography (ICG)-based method for detecting Leptospira antigen in the urine of patients and animals. We used the 1H6 monoclonal antibody (MAb), which is specific to the lipopolysaccharide (LPS) that is common among Leptospira spp. The MAb was coupled to 40-nm-diameter colloidal gold, and the amounts of labeled antibody and immobilized antibody were 23 μg and 2 μg per test, respectively. Several strains of Leptospira and other bacterial species were used to evaluate the sensitivities and specificities of the assays we developed. The detection limit of the assays was 10(6) cells/ml when disrupted whole bacterial cells were used. The assays were Leptospira specific since they did not cross-react with non-Leptospira bacteria used in the study. Application of diagnostic assays was done on the urine samples of 46 Leptospira-infected hamsters, 44 patients with suspected leptospirosis, and 14 healthy individuals. Pretreatment of the urine samples by boiling and centrifugation (for ultrafiltration and concentration) eliminated nonspecific reactions that occurred in the assay. The sensitivity and specificity of the ICG-based lateral flow assay (LFA) were 89% and 87%, respectively, which were higher than those of the dipstick assay, which were 80% and 74%, respectively. In summary, this ICG-based LFA can be used as an alternative diagnostic assay for leptospirosis. Further development is still necessary to improve the assay.
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