ArticleBiology of reproduction2013
Human lactate dehydrogenase A (LDHA) rescues mouse Ldhc-null sperm function.
Article in Biology of reproduction, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Proteomic biomarkers in seminal plasma as predictors of reproductive potential in azoospermic men.Frontiers in endocrinology · 2024Article
- Ldha-Dependent Metabolic Programs in Sertoli Cells Regulate Spermiogenesis in Mouse Testis.Biology · 2022Article
- Cancer-testis antigen lactate dehydrogenase C4 as a novel biomarker of male infertility and cancer.Frontiers in oncology · 2022Review
- Capacitation promotes a shift in energy metabolism in murine sperm.Frontiers in cell and developmental biology · 2022Article
- Identification of testicular Foxq1 as a critical modulator of lactate metabolism in mouse Sertoli cells.Histochemistry and cell biology · 2021Article
- Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis).Molecular reproduction and development · 2021Article
- Uncovering sperm metabolome to discover biomarkers for bull fertility.BMC genomics · 2019Article
- The protein phosphatase isoform PP1γ1 substitutes for PP1γ2 to support spermatogenesis but not normal sperm function and fertility†.Biology of reproduction · 2019Article
- The Warburg Effect Is Associated With Tumor Aggressiveness in Testicular Germ Cell Tumors.Frontiers in endocrinology · 2019Article
- Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels.PloS one · 2017Article
- Comparative expression profiling of testis-enriched genes regulated during the development of spermatogonial cells.PloS one · 2017Article
- Lactate Dehydrogenase C Produces S-2-Hydroxyglutarate in Mouse Testis.ACS chemical biology · 2016Article
- Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.BMC nephrology · 2016Article
- Differences in ATP Generation Via Glycolysis and Oxidative Phosphorylation and Relationships with Sperm Motility in Mouse Species.The Journal of biological chemistry · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
By targeted disruption of the lactate dehydrogenase c (Ldhc) gene, we demonstrated that spermatozoa require Ldhc for capacitation, motility, and fertilizing capacity. Ldhc expression is restricted to the developing germ cells that, however, are apparently not compromised by the lack of the LDHC isozyme. Because LDHC is abundant in spermatozoa that utilize aerobic glycolysis for energy requirements, its main function was presumed to be the interconversion of pyruvate to lactate with the concomitant oxidation/reduction of NADH to NAD(+). We found that sperm without LDHC were still able to convert lactate to pyruvate as mediated by LDHA that is tightly bound to the fibrous sheath. It was assumed that the level of glycolysis was insufficient to power motility and the subsequent fertilizing capacity of the mutated sperm. To investigate whether LDHC possesses certain unique characteristics essential for fertility, human LDHA was introduced as a transgene to Ldhc-null mice. We report here that the exogenous LDHA rescued the phenotype of the Ldhc-null males. Sperm from the LDHA transgenic males with the Ldhc deletion (LDHA(+)/Ldhc(-/-)) are motile, capable of protein tyrosine phosphorylation, and able to fertilize, thus restoring these properties to LDHC-null sperm. However, the lactate and ATP levels in the rescued sperm did not differ significantly from sperm lacking LDHC. We suggest that it is the localization of the transgene to the sperm cytosol that is mainly responsible for restoration of sperm function and fertility.
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