A new approach for aggregation of Paramecium caudatum by nitric oxide.

Manizheh Karami, Seyed Sajad Shahrokhi, Bahram Kazemi, Seyedeh Samaneh Moezzi

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Article in Iranian journal of microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

–field-weighted citation impact

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1 citing paper in PubMed.

Interaction of nicotine with morphine potency inHeliyon · 2019
Article

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5 · Who and what money

Authors and funding

4 authors.

Manizheh KaramiDepartment of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.

Seyed Sajad Shahrokhi

Bahram Kazemi

Seyedeh Samaneh Moezzi

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesNitric oxide (NO) plays a role in thermoregulation and growth of protozoa. This work aimed to add the molecule NO in physiology of protozoa in contact with abused narcotic substances. MATERIALS AND

methodsA sedative drug, morphine, was infused into a cell chamber containing Paramecia. The cell response to the drug was recorded promptly after drug infusion using a potency protocol provided for the first time at this laboratory. A precursor of NO, L-arginine, was treated jointly with drug to involve the NO system in protozoan performance to drug exposure. Marking of NADPH-diaphorase (NADPH-d) was followed to provide data to explain the mechanisms.

resultsMorphine particularly (0.5 to 60 µg/µ1) aggregated the Paramecia. The infusion of L-arginine (1 to 8 µg/µ1) together with morphine potentiated this effect, though, pre-usage of L-NAME (1 to 8 µg/µ1), a blocker of NO production, reversed the response. Notably the activation of NADPH-d in solely morphine or L-arginine plus morphine samples was revealed. However, the expression of marker was attenuated upon pre-infusion with L-NAME.

conclusionThis study introduces a new approach to involve NO in physiology of aggregation of Paramecia following exposure to the misused sedative drug, morphine.

Indexed as

AggregationMorphineNADPH-diaphoraseNitric oxideParamecium

Identifiers

PMID23467065

PMCPMC3577564

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