ArticleIranian journal of microbiology2013
Molecular characterization of class 1 integrons and gene cassettes in multidrug resistant (MDR) Klebsiella spp. isolated from hospitalized and outpatients in Iran, 2009.
Article in Iranian journal of microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Clonal dissemination of carbapenem-resistant Klebsiella pneumoniae in outpatients as fecal-carriages: predominance of the high-risk clone ST231.BMC infectious diseases · 2025Article
- Molecular detection of Class 1, 2, and 3 integrons in hypervirulent and classicHealth science reports · 2024Article
- Molecular Characterization of Antibiotic Resistance and Genetic Diversity ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2022Article
- Prevalence of Extended-Spectrum β-lactamase and Integron Gene Carriage in Multidrug-ResistantIranian journal of medical sciences · 2020Article
- Molecular characterization of class 1, 2 and 3 integrons in clinical multi-drug resistantAntimicrobial resistance and infection control · 2019Article
- Gene cassettes of class I integron-associated with antimicrobial resistance in isolates ofIranian journal of microbiology · 2018Article
- Nosocomial emerging of (VIM1) carbapenemase-producing isolates of Klebsiella pneumoniae in North of Iran.Iranian journal of microbiology · 2015Article
- The function of integron-associated gene cassettes in Vibrio species: the tip of the iceberg.Frontiers in microbiology · 2013Review
- Investigation of Integron-Associated Resistance Gene Cassettes in Urinary Isolates ofAvicenna journal of medical biotechnologyArticle
Corrections and comments
- Erratum issuedMousavi, Seyed Fazlollah [added]
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivesKlebsiella species are of the most common bacteria involved in nosocomial and urinary tract infections. Genetic elements such as class 1 integrons have an important role in the resistance development. In this study, the share of class 1 integrons, the genetic characterization of the integron cassettes and PFGE profiles of the clinical Klebsiella isolates are evaluated in Besat University hospital of Sanandaj, Iran.
methodsIsolates from 17890 clinical specimens were identified by API20E. Antibiotic susceptibility testing and MIC were done for MDR isolates. For investigating class 1 integrons and gene cassettes, PCR by intI1 integrase and 5'-CS/3'-CS were performed. Integrated gene cassettes were analyzed by PCR-RFLP and sequencing. Pulsed-Field Gel Electrophoresis was carried out for studying of clonality outbreak of isolates.
resultsThirty five Klebsiella spp. were isolated and included 29 K. pneumoniae and 6 K. oxytoca. All the isolates were susceptible to carbapenems while other antibiotics showed high resistant profile. In all Klebsiella spp. PCR for intI1 integrase and 5'-CS/3'-CS were positive (100%). Sequencing for prevalent bands of internal variable regions between 5'-CS/3'-CS showed arr-5, orfD-aacA4 and aad5- dfrA17. PFGE Analysis showed 18 clusters in K. pneumoniae with clonality relatedness in some cases but no relatedness among K. oxytoca isolates.
conclusionHigh prevalence of class 1 integron carrying gene cassettes confirms that integron-mediated antimicrobial gene cassettes are important in Klebsiella spp. resistance profile. Clone diffusions of MDR Klebsiella spp. which harbor class 1 integrons have threaten the potential in the resistance development in our clinical settings.
Indexed as
Identifiers
23466743PMC3577558W2128881971What OpenQuestion holds
Registered trials
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