ArticleThe EMBO journal2013
PDK1 regulates VDJ recombination, cell-cycle exit and survival during B-cell development.
Article in The EMBO journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- A Microbiota- and IL-15-Dependent Innate-Like B Cell Progenitor Expressing E4BP4.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The significance of PAX5 in Merkel cell carcinoma.The Journal of pathology · 2025Article
- The mTORC2 signaling network: targets and cross-talks.The Biochemical journal · 2024Review
- The function of serine/threonine-specific protein kinases in B cells.Frontiers in immunology · 2024Review
- miR-582 negatively regulates pre-B cell proliferation and survival through targeting Hif1α and Rictor.Cell death & disease · 2022Article
- PDK1 Is Required for Maintenance of CD4Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Article
- PDK1 regulates the survival of the developing cortical interneurons.Molecular brain · 2020Article
- [The role of PDK1 in the transition of endothelial to hematopoietic cells].Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi · 2018Article
- Discrete roles and bifurcation of PTEN signaling and mTORC1-mediated anabolic metabolism underlie IL-7-driven B lymphopoiesis.Science advances · 2018Article
- Article
- PDK1 plays a vital role on hematopoietic stem cell function.Scientific reports · 2017Article
- Review
- Characterisation of mice lacking all functional isoforms of the pro-survival BCL-2 family member A1 reveals minor defects in the haematopoietic compartment.Cell death and differentiation · 2017Article
- Conditional Disruption of Raptor Reveals an Essential Role for mTORC1 in B Cell Development, Survival, and Metabolism.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- The PI3K pathway in B cell metabolism.Critical reviews in biochemistry and molecular biology · 2016Review
- The PDK1-Rsk Signaling Pathway Controls Langerhans Cell Proliferation and Patterning.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- PDK1 orchestrates early NK cell development through induction of E4BP4 expression and maintenance of IL-15 responsiveness.The Journal of experimental medicine · 2015Article
- Dichloroacetate, the Pyruvate Dehydrogenase Complex and the Modulation of mESC Pluripotency.PloS one · 2015Article
- PDK1 regulates B cell differentiation and homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Phosphoinositide-dependent kinase-1 (PDK1) controls the activation of a subset of AGC kinases. Using a conditional knockout of PDK1 in haematopoietic cells, we demonstrate that PDK1 is essential for B cell development. B-cell progenitors lacking PDK1 arrested at the transition of pro-B to pre-B cells, due to a cell autonomous defect. Loss of PDK1 decreased the expression of the IgH chain in pro-B cells due to impaired recombination of the IgH distal variable segments, a process coordinated by the transcription factor Pax5. The expression of Pax5 in pre-B cells was decreased in PDK1 knockouts, which correlated with reduced expression of the Pax5 target genes IRF4, IRF8 and Aiolos. As a result, Ccnd3 is upregulated in PDK1 knockout pre-B cells and they have an impaired ability to undergo cell-cycle arrest, a necessary event for Ig light chain rearrangement. Instead, these cells underwent apoptosis that correlated with diminished expression of the pro-survival gene Bcl2A1. Reintroduction of both Pax5 and Bcl2A1 together into PDK1 knockout pro-B cells restored their ability to differentiate in vitro into mature B cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.