ArticleMutagenesis2013
Thirdhand smoke causes DNA damage in human cells.
Article in Mutagenesis, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
83 citing papers in PubMed, 1 synthesis or guideline pooled it, 167 citations in OpenAlex.
- Analysis of Assessment Methods for Detecting Nicotine Residue and Its Impact on Humans: A Systematic Review.International journal of environmental research and public health · 2025Pooled it
- Experimental Acute Exposure to Thirdhand Smoke and Changes in the Human Nasal Epithelial Transcriptome: A Randomized Clinical Trial.JAMA network open · 2019Trial
- Parental Smoking and E-cigarette Use in Homes and Cars.Pediatrics · 2019Trial
- Thirdhand Smoke in the Homes of Medically Fragile Children: Assessing the Impact of Indoor Smoking Levels and Smoking Bans.Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco · 2016Trial
- Thirdhand smoke beliefs of parents.Pediatrics · 2014Trial
- Beliefs about thirdhand smoke and voluntary smoking bans in homes and cars: a cross-sectional study in Türkiye, 2023.BMC public health · 2026Article
- A Critical Perspective on the Role of Thirdhand Smoke in Tumorigenesis: Initiator or Promoter.Environment & health (Washington, D.C.) · 2026Review
- Integrated network toxicology and multi-omics analysis reveals the role of tobacco-specific carcinogen NNK in idiopathic pulmonary fibrosis pathogenesis.Respiratory research · 2026Article
- Low dose thirdhand smoke exposure enhances platelet functional responses in mice.Experimental biology and medicine (Maywood, N.J.) · 2026Article
- Thirdhand Smoke and Neonatal/Pediatric Health: A Scoping Review on Nursing Implications.Healthcare (Basel, Switzerland) · 2025Review
- Memory under siege: the cognitive costs of smoking and vaping.Brain, behavior, & immunity - health · 2025Review
- Associations of Involuntary Smoking with Non-Suicidal Self-Injury and Suicidal Behaviors in Early Adulthood.Toxics · 2025Article
- Awareness of third-hand smoke: A cross-sectional study among medical students at Qassim University.Journal of family medicine and primary care · 2025Article
- Perceptions of Patients with Respiratory Disorders About Environmental Smoke.Sisli Etfal Hastanesi tip bulteni · 2025Article
- Associations of environmental tobacco smoke with ADHD and executive function in early adulthood: results from a cross-sectional study.Frontiers in psychiatry · 2025Article
- Oxidative DNA Damage and Arterial Hypertension in Light of Current ESC Guidelines.International journal of molecular sciences · 2024Review
- Third-Hand Smoke Exacerbates HTurkish journal of pharmaceutical sciences · 2024Article
- Policy-relevant differences between secondhand and thirdhand smoke: strengthening protections from involuntary exposure to tobacco smoke pollutants.Tobacco control · 2024Article
- Third-hand smoke perception and awareness among medical students: a survey study.Scientific reports · 2024Article
- IVF cycle safety when a positive passive air sampling occurs under laminar flow hood in absence of a detectable contamination in the embryo culture.Frontiers in cell and developmental biology · 2024Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 5 institutions in 1 country.
Funding
Abstract
Exposure to thirdhand smoke (THS) is a newly described health risk. Evidence supports its widespread presence in indoor environments. However, its genotoxic potential, a critical aspect in risk assessment, is virtually untested. An important characteristic of THS is its ability to undergo chemical transformations during aging periods, as demonstrated in a recent study showing that sorbed nicotine reacts with the indoor pollutant nitrous acid (HONO) to form tobacco-specific nitrosamines (TSNAs) such as 4-(methylnitrosamino)-4-(3-pyridyl)butanal (NNA) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). The goal of this study was to assess the genotoxicity of THS in human cell lines using two in vitro assays. THS was generated in laboratory systems that simulated short (acute)- and long (chronic)-term exposures. Analysis by liquid chromatography-tandem mass spectrometry quantified TSNAs and common tobacco alkaloids in extracts of THS that had sorbed onto cellulose substrates. Exposure of human HepG2 cells to either acute or chronic THS for 24h resulted in significant increases in DNA strand breaks in the alkaline Comet assay. Cell cultures exposed to NNA alone showed significantly higher levels of DNA damage in the same assay. NNA is absent in freshly emitted secondhand smoke, but it is the main TSNA formed in THS when nicotine reacts with HONO long after smoking takes place. The long amplicon-quantitative PCR assay quantified significantly higher levels of oxidative DNA damage in hypoxanthine phosphoribosyltransferase 1 (HPRT) and polymerase β (POLB) genes of cultured human cells exposed to chronic THS for 24h compared with untreated cells, suggesting that THS exposure is related to increased oxidative stress and could be an important contributing factor in THS-mediated toxicity. The findings of this study demonstrate for the first time that exposure to THS is genotoxic in human cell lines.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.