ArticlePloS one2013
The internal transcribed spacer (ITS) region and trnH-psbA [corrected] are suitable candidate loci for DNA barcoding of tropical tree species of India.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 37 papers.
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37 citing papers in PubMed.
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- Ability to use ITS and rbcL sequencing for determination of the genetic diversity and relationships among olive (3 Biotech · 2025Article
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- Internal transcribed spacer (ITS): The powerful DNA barcode and phylogenetic marker for successful authentication of Withania somnifera.Molecular biology reports · 2024Article
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- DNA barcoding in herbal medicine: Retrospective and prospective.Journal of pharmaceutical analysis · 2023Review
- A DNA barcode reference library for CITES listed MalagasyEcology and evolution · 2023Article
- Authentication ofPlants (Basel, Switzerland) · 2022Article
- Advancements and future prospective of DNA barcodes in the herbal drug industry.Frontiers in pharmacology · 2022Review
- DNA barcoding of some medicinally important plant species of Lamiaceae family in India.Molecular biology reports · 2021Article
- Chemical composition and biological effects of kratom (Mitragyna speciosa): In vitro studies with implications for efficacy and drug interactions.Scientific reports · 2020Article
- Molecular identification and evolutionary relationships between the subspecies of Musa by DNA barcodes.BMC genomics · 2020Article
- DNA barcoding supports existence of morphospecies complex in endemic bamboo genusJournal of genetics · 2020Article
- Combining DNA Barcoding and HPLC Fingerprints to Trace Species of an Important Traditional Chinese Medicine Fritillariae Bulbus.Molecules (Basel, Switzerland) · 2019Article
- Diversification of African Tree Legumes in Miombo-Mopane Woodlands.Plants (Basel, Switzerland) · 2019Article
- Karyological Analysis and DNA Barcoding of Pompia Citron: A First Step toward the Identification of Its Relatives.Plants (Basel, Switzerland) · 2019Article
- Development of conventional PCR and real-time PCR assays to discriminate the origins of Chinese pepper oil and herbal materials from Zanthoxylum.Journal of the science of food and agriculture · 2019Article
- DNA barcoding and TLC as tools to properly identify natural populations of the Mexican medicinal species Galphimia glauca Cav.PloS one · 2019Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDNA barcoding as a tool for species identification has been successful in animals and other organisms, including certain groups of plants. The exploration of this new tool for species identification, particularly in tree species, is very scanty from biodiversity-rich countries like India. rbcL and matK are standard barcode loci while ITS, and trnH-psbA are considered as supplementary loci for plants. METHODOLOGY AND PRINCIPAL
findingsPlant barcode loci, namely, rbcL, matK, ITS, trnH-psbA, and the recently proposed ITS2, were tested for their efficacy as barcode loci using 300 accessions of tropical tree species. We tested these loci for PCR, sequencing success, and species discrimination ability using three methods. rbcL was the best locus as far as PCR and sequencing success rate were concerned, but not for the species discrimination ability of tropical tree species. ITS and trnH-psbA were the second best loci in PCR and sequencing success, respectively. The species discrimination ability of ITS ranged from 24.4 percent to 74.3 percent and that of trnH-psbA was 25.6 percent to 67.7 percent, depending upon the data set and the method used. matK provided the least PCR success, followed by ITS2 (59. 0%). Species resolution by ITS2 and rbcL ranged from 9.0 percent to 48.7 percent and 13.2 percent to 43.6 percent, respectively. Further, we observed that the NCBI nucleotide database is poorly represented by the sequences of barcode loci studied here for tree species.
conclusionAlthough a conservative approach of a success rate of 60-70 percent by both ITS and trnH-psbA may not be considered as highly successful but would certainly help in large-scale biodiversity inventorization, particularly for tropical tree species, considering the standard success rate of plant DNA barcode program reported so far. The recommended matK and rbcL primers combination may not work in tropical tree species as barcode markers.
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