ArticleThe Journal of biological chemistry2013
Oligomeric structure and functional characterization of Caenorhabditis elegans Innexin-6 gap junction protein.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 37 citations in OpenAlex.
- In situ structure of a gap junction-stomatin complex.Science advances · 2025Article
- Configuration of electrical synapses filters sensory information to drive behavioral choices.Cell · 2025Article
- Unnexin is a protein subunit of a large-pore channel expressed by unicellular organisms.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Independent Innexin Radiation Shaped Signaling in Ctenophores.Molecular biology and evolution · 2023Article
- Gap junctions mediate discrete regulatory steps during fly spermatogenesis.PLoS genetics · 2022Article
- cAMP controls a trafficking mechanism that maintains the neuron specificity and subcellular placement of electrical synapses.Developmental cell · 2021Article
- Molecular and morphological approach to study the innexin gap junctions inOpen biology · 2021Article
- Uncovering the genetic blueprint of theProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Gap junctions: historical discoveries and new findings in theBiology open · 2020Review
- Cryo-EM structures of undocked innexin-6 hemichannels in phospholipids.Science advances · 2020Article
- Gap Junction Channels of Innexins and Connexins: Relations and Computational Perspectives.International journal of molecular sciences · 2019Review
- Control of oocyte meiotic maturation in C. elegans.Seminars in cell & developmental biology · 2018Review
- Structure of an innexin gap junction channel and cryo-EM sample preparation.Microscopy (Oxford, England) · 2017Review
- Gap junctions in C. elegans: Their roles in behavior and development.Developmental neurobiology · 2017Review
- A structural and functional comparison of gap junction channels composed of connexins and innexins.Developmental neurobiology · 2017Review
- Characterization of the Tetraspan Junctional Complex (4JC) superfamily.Biochimica et biophysica acta. Biomembranes · 2017Article
- Atomic structure of the innexin-6 gap junction channel determined by cryo-EM.Nature communications · 2016Article
- Tryptophan Scanning Reveals Dense Packing of Connexin Transmembrane Domains in Gap Junction Channels Composed of Connexin32.The Journal of biological chemistry · 2015Article
- Mechanosensitive unpaired innexin channels in C. elegans touch neurons.American journal of physiology. Cell physiology · 2014Article
- The pannexins: past and present.Frontiers in physiology · 2014Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Innexin is the molecular component of invertebrate gap junctions. Here we successfully expressed and purified Caenorhabditis elegans innexin-6 (INX-6) gap junction channels and characterized the molecular dimensions and channel permeability using electron microscopy (EM) and microinjection of fluorescent dye tracers, respectively. Negative staining and thin-section EM of isolated INX-6 gap junction membranes revealed a loosely packed hexagonal lattice and a greater cross-sectional width than that of connexin26 and connexin43 (Cx43)-GFP. In gel filtration analysis, the elution profile of purified INX-6 channels in dodecyl maltoside solution exhibited a peak at ∼400 kDa that was shifted to ∼800 kDa in octyl glucose neopentyl glycol. We also obtained the class averages of purified INX-6 channels from these peak fractions by single particle analysis. The class average from the ∼800-kDa fraction showed features of the junction form with a longitudinal height of 220 Å, a channel diameter of 110 Å in the absence of detergent micelles, and an extracellular gap space of 60 Å, whereas the class averages from the ∼400-kDa fraction showed diameters of up to 140 Å in the presence of detergent micelles. These findings indicate that the purified INX-6 channels are predominantly hemichannels in dodecyl maltoside and docked junction channels in octyl glucose neopentyl glycol. Dye transfer experiments revealed that the INX-6-GFP-His channels are permeable to 3- and 10-kDa tracers, whereas no significant amounts of these tracers passed through the Cx43-GFP channels. Based on these findings, INX-6 channels have a larger overall structure and greater permeability than connexin channels.
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