ArticleMolecular and cellular biology2013
Developmentally programmed 3' CpG island methylation confers tissue- and cell-type-specific transcriptional activation.
Article in Molecular and cellular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 50 citations in OpenAlex.
- Weaning drives microbiome-mediated epigenetic regulation to shape immune memory in mice.Nature microbiology · 2026Article
- Functional characterization of age-dependent p16 epimutation reveals biological drivers and therapeutic targets for colorectal cancer.Journal of experimental & clinical cancer research : CR · 2023Article
- The effects of methionine administration during the beginning postnatal days on the ovarian structures in adult rats.Veterinary medicine and science · 2022Article
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- DNA methylation in AgRP neurons regulates voluntary exercise behavior in mice.Nature communications · 2019Article
- Epigenetics and Female Reproductive Aging.Frontiers in endocrinology · 2019Review
- Identification and analysis of key genes in osteosarcoma using bioinformatics.Oncology letters · 2018Article
- Adiponectin is required for maintaining normal body temperature in a cold environment.BMC physiology · 2017Article
- Structural Basis for the Versatile and Methylation-Dependent Binding of CTCF to DNA.Molecular cell · 2017Article
- Energy Balance Modulation Impacts Epigenetic Reprogramming, ERα and ERβ Expression, and Mammary Tumor Development in MMTV-neu Transgenic Mice.Cancer research · 2017Article
- Whole genome DNA methylation: beyond genes silencing.Oncotarget · 2017Review
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- Transcription factors as readers and effectors of DNA methylation.Nature reviews. Genetics · 2016Review
- Lactase nonpersistence is directed by DNA-variation-dependent epigenetic aging.Nature structural & molecular biology · 2016Article
- Tet1 Oxidase Regulates Neuronal Gene Transcription, Active DNA Hydroxy-methylation, Object Location Memory, and Threat Recognition Memory.Neuroepigenetics · 2015Article
- Postnatal epigenetic regulation of intestinal stem cells requires DNA methylation and is guided by the microbiome.Genome biology · 2015Article
- Post-transcriptional gene silencing, transcriptional gene silencing and human immunodeficiency virus.World journal of virology · 2015Review
- Article
- Integrative analysis of methylome and transcriptome in human blood identifies extensive sex- and immune cell-specific differentially methylated regions.Epigenetics · 2015Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
During development, a small but significant number of CpG islands (CGIs) become methylated. The timing of developmentally programmed CGI methylation and associated mechanisms of transcriptional regulation during cellular differentiation, however, remain poorly characterized. Here, we used genome-wide DNA methylation microarrays to identify epigenetic changes during human embryonic stem cell (hESC) differentiation. We discovered a group of CGIs associated with developmental genes that gain methylation after hESCs differentiate. Conversely, erasure of methylation was observed at the identified CGIs during subsequent reprogramming to induced pluripotent stem cells (iPSCs), further supporting a functional role for the CGI methylation. Both global gene expression profiling and quantitative reverse transcription-PCR (RT-PCR) validation indicated opposing effects of CGI methylation in transcriptional regulation during differentiation, with promoter CGI methylation repressing and 3' CGI methylation activating transcription. By studying diverse human tissues and mouse models, we further confirmed that developmentally programmed 3' CGI methylation confers tissue- and cell-type-specific gene activation in vivo. Importantly, luciferase reporter assays provided evidence that 3' CGI methylation regulates transcriptional activation via a CTCF-dependent enhancer-blocking mechanism. These findings expand the classic view of mammalian CGI methylation as a mechanism for transcriptional silencing and indicate a functional role for 3' CGI methylation in developmental gene regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.