ArticlePLoS genetics2013
Only one isoform of Drosophila melanogaster CTP synthase forms the cytoophidium.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 63 citations in OpenAlex.
- The Impact of Developmental and Metabolic Cues on Cytoophidium Formation.International journal of molecular sciences · 2024Review
- Differential Cytoophidium Assembly betweenInternational journal of molecular sciences · 2024Article
- Dynamic Cytoophidia during Late-StageInternational journal of molecular sciences · 2024Article
- Connecting Hippo Pathway and Cytoophidia inInternational journal of molecular sciences · 2024Article
- Cytoophidia Maintain the Integrity ofInternational journal of molecular sciences · 2022Article
- CTP synthase: the hissing of the cellular serpent.Histochemistry and cell biology · 2022Review
- Cytoophidia coupling adipose architecture and metabolism.Cellular and molecular life sciences : CMLS · 2022Article
- Motor defects in a Drosophila model for spinal muscular atrophy result from SMN depletion during early neurogenesis.PLoS genetics · 2022Article
- Prebiotic Chemistry: The Role of Trimetaphosphate in Prebiotic Chemical Evolution.Frontiers in chemistry · 2022Review
- Highly effective proximate labeling in Drosophila.G3 (Bethesda, Md.) · 2021Article
- ASNS disruption shortens CTPS cytoophidia in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2021Article
- The TOR pathway modulates cytoophidium formation inThe Journal of biological chemistry · 2019Article
- Temperature-sensitive cytoophidium assembly in Schizosaccharomyces pombe.Journal of genetics and genomics = Yi chuan xue bao · 2019Article
- Ectopic miR-975 induces CTP synthase directed cell proliferation and differentiation in Drosophila melanogaster.Scientific reports · 2019Article
- Cytoophidia respond to nutrient stress in Drosophila.Experimental cell research · 2019Article
- mTOR-S6K1 pathway mediates cytoophidium assembly.Journal of genetics and genomics = Yi chuan xue bao · 2019Article
- Regulation of Cellular Metabolism through Phase Separation of Enzymes.Biomolecules · 2018Review
- Characterization of filament-forming CTP synthases from Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2018Article
- High level of CTP synthase induces formation of cytoophidia in cortical neurons and impairs corticogenesis.Histochemistry and cell biology · 2018Article
- Critical roles of CTP synthase N-terminal in cytoophidium assembly.Experimental cell research · 2017Article
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Authors and funding
2 authors at 2 institutions in 1 country.
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Abstract
CTP synthase is an essential enzyme that plays a key role in energy metabolism. Several independent studies have demonstrated that CTP synthase can form an evolutionarily conserved subcellular structure termed cytoophidium. In budding yeast, there are two isoforms of CTP synthase and both isoforms localize in cytoophidium. However, little is known about the distribution of CTP synthase isoforms in Drosophila melanogaster. Here, we report that three transcripts generated at the CTP synthase gene locus exhibit different expression profiles, and three isoforms encoded by this gene locus show a distinct subcellular distribution. While isoform A localizes in the nucleus, isoform B distributes diffusely in the cytoplasm, and only isoform C forms the cytoophidium. In the two isoform C-specific mutants, cytoophidia disappear in the germline cells. Although isoform A does not localize to the cytoophidium, a mutation disrupting mostly isoform A expression results in the disassembly of cytoophidia. Overexpression of isoform C can induce the growth of the cytoophidium in a cell-autonomous manner. Ectopic expression of the cytoophidium-forming isoform does not cause any defect in the embryos. In addition, we identify that a small segment at the amino terminus of isoform C is necessary but not sufficient for cytoophidium formation. Finally, we demonstrate that an excess of the synthetase domain of CTP synthase disrupts cytoophidium formation. Thus, the study of multiple isoforms of CTP synthase in Drosophila provides a good opportunity to dissect the biogenesis and function of the cytoophidum in a genetically tractable organism.
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