ArticleAntimicrobial agents and chemotherapy2013
Real-time microscopic observation of Candida biofilm development and effects due to micafungin and fluconazole.
Article in Antimicrobial agents and chemotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- A quantitative framework for multiscale analysis ofBiofilm · 2026Article
- In vitro evaluation of antimicrobial activity of andrographolide against mixed biofilms of Candida albicans and Staphylococcus aureus.BMC complementary medicine and therapies · 2026Article
- Natural-Compound Adjuvants Dismantle Candida Biofilms: Mechanisms, Design Rules, and Biofilm-Aware Pharmacology.Current microbiology · 2026Review
- Sunflower Oil and Cholesterol Nanoemulsion: A Novel Carrier for Micafungin to Combat Multi-ResistantPathogens (Basel, Switzerland) · 2024Article
- Real-time monitoring of biofilm growth identifies andrographolide as a potent antifungal compound eradicatingBiofilm · 2023Article
- Understanding the diagnosis of catheter-related bloodstream infection: real-time monitoring of biofilm growth dynamics using time-lapse optical microscopy.Frontiers in cellular and infection microbiology · 2023Article
- Bacterial Interactions in the Context of Chronic Wound Biofilm: A Review.Microorganisms · 2022Review
- Association of different Candida species with catheter-related candidemia, and the potential antifungal treatments against their adhesion properties and biofilm-forming capabilities.Journal of clinical laboratory analysis · 2021Article
- Candidemia: Evolution of Drug Resistance and Novel Therapeutic Approaches.Infection and drug resistance · 2021Review
- Effects of farnesol and lyticase on the formation ofVeterinary world · 2020Article
- Antifungal Activity of the Natural Coumarin Scopoletin Against Planktonic Cells and Biofilms From a Multidrug-ResistantFrontiers in microbiology · 2020Article
- Application of 460 nm visible light for the elimination of Candida albicans in vitro and in vivo.Molecular medicine reports · 2018Article
- In vitro activity of micafungin against biofilms of Candida albicans, Candida glabrata, and Candida parapsilosis at different stages of maturation.Folia microbiologica · 2018Article
- CD101, a Novel Echinocandin, Possesses Potent Antibiofilm Activity against Early and Mature Candida albicans Biofilms.Antimicrobial agents and chemotherapy · 2018Article
- Interaction of Candida albicans with host cells: virulence factors, host defense, escape strategies, and the microbiota.Journal of microbiology (Seoul, Korea) · 2016Review
- Candida Biofilms: Development, Architecture, and Resistance.Microbiology spectrum · 2015Review
- The Role of Antifungals against Candida Biofilm in Catheter-Related Candidemia.Antibiotics (Basel, Switzerland) · 2014Review
- Recent insights into Candida albicans biofilm resistance mechanisms.Current genetics · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the process of Candida biofilm development and the effects of antifungal agents on biofilms, we analyzed real-time data comprising time-lapse images taken at times separated by brief intervals. The growth rate was calculated by measuring the change of biofilm thickness every hour. For the antifungal study, 5-h-old biofilms of Candida albicans were treated with either micafungin (MCFG) or fluconazole (FLCZ). MCFG began to suppress biofilm growth a few minutes after the initiation of the treatment, and this effect was maintained over the course of the observation period. In contrast, the suppressive effects of FLCZ on biofilm growth took longer to manifest: biofilms grew in the first 5 h after treatment, and then their growth was suppressed over the next 10 h, finally producing results similar to those observed with MCFG. MCFG was also involved in the disruption of cells in the biofilms, releasing string-like structures (undefined extracellular component) from the burst hyphae. Thus, MCFG inhibited the detachment of yeast cell clusters from the tips of hyphae. In contrast, FLCZ did not disrupt biofilm cells. MCFG also showed fast antifungal activity against Candida parapsilosis biofilms. In conclusion, our results show that inhibition of glucan synthesis due to MCFG contributed not only to fungicidal activity but also to the immediate suppression of biofilm growth, while FLCZ suppressed growth by inhibiting ergosterol synthesis. Therefore, those characteristic differences should be considered when treating clinical biofilm infections.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.