ArticleThe American journal of tropical medicine and hygiene2013
Molecular and biological diagnostic tests for monitoring benzimidazole resistance in human soil-transmitted helminths.
Article in The American journal of tropical medicine and hygiene, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 2 syntheses or guidelines pooled it, 84 citations in OpenAlex.
- Pooled it
- Benzimidazole Resistance-Associated Mutations in the β-tubulin Gene of Hookworms: A Systematic Review.Parasitology research · 2024Pooled it
- Therapeutic efficacy of albendazole against soil-transmitted helminthiasis in children measured by five diagnostic methods.PLoS neglected tropical diseases · 2019Trial
- Comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: A Starworms study protocol.PLoS neglected tropical diseases · 2018Trial
- Thermosensory behaviors of the free-living life stages of Strongyloides species support parasitism in tropical environments.PLoS neglected tropical diseases · 2024Article
- Thermosensory behaviors of the free-living life stages ofbioRxiv : the preprint server for biology · 2024Article
- New Insights on Tools for Detecting β-Tubulin Polymorphisms inAnimals : an open access journal from MDPI · 2024Article
- The equine ascarids: resuscitating historic model organisms for modern purposes.Parasitology research · 2022Review
- Identification of key interactions of benzimidazole resistance-associated amino acid mutations in Ascaris β-tubulins by molecular docking simulations.Scientific reports · 2022Article
- Application of multiplex amplicon deep-sequencing (MAD-seq) to screen for putative drug resistance markers in the Necator americanus isotype-1 β-tubulin gene.Scientific reports · 2022Article
- Using newly optimized genetic tools to probe Strongyloides sensory behaviors.Molecular and biochemical parasitology · 2022Review
- The neural basis of heat seeking in a human-infective parasitic worm.Current biology : CB · 2022Article
- Improving stool sample processing and pyrosequencing for quantifying benzimidazole resistance alleles in Trichuris trichiura and Necator americanus pooled eggs.Parasites & vectors · 2021Article
- Characterization of the β-tubulin gene family in Ascaris lumbricoides and Ascaris suum and its implication for the molecular detection of benzimidazole resistance.PLoS neglected tropical diseases · 2021Article
- Challenges and opportunities for the adoption of molecular diagnostics for anthelmintic resistance.International journal for parasitology. Drugs and drug resistance · 2020Article
- The role of carbon dioxide in nematode behaviour and physiology.Parasitology · 2020Review
- Role of DNA-detection-based tools for monitoring the soil-transmitted helminth treatment response in drug-efficacy trials.PLoS neglected tropical diseases · 2020Article
- Absence of mutations associated with resistance to benzimidazole in the beta-tubulin gene of Ascaris suum.Revista da Sociedade Brasileira de Medicina Tropical · 2020Article
- Multiple drug resistance in the canine hookworm Ancylostoma caninum: an emerging threat?Parasites & vectors · 2019Article
- Oxamniquine resistance alleles are widespread in Old World Schistosoma mansoni and predate drug deployment.PLoS pathogens · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In endemic countries with soil-transmitted helminths mass drug administration with albendazole or mebendazole are being implemented as a control strategy. However, it is well known in veterinary helminths that the use of the same benzimidazole drugs can place selection on the β-tubulin gene, leading to resistance. Given the concern that resistance could arise in human soil-transmitted helminths, there is an urgent need to develop accurate diagnostic tools for monitoring resistance. In this study, we developed molecular assays to detect putative resistance genetic changes in Ascaris lumbricoides, Trichuris trichiura, and hookworms, and we optimized an egg hatch assay for the canine hookworm Ancylostoma caninum and applied it to Necator americanus. Both assays were tested on field samples. The molecular assays demonstrated their reproducibility and capacity to detect the presence of worms carrying putative resistance-associated genetic changes. However, further investigations are needed to validate our molecular and biological tests on additional field isolates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.