ArticleGene2013
RNA sequencing and transcriptomal analysis of human monocyte to macrophage differentiation.
Article in Gene, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages.International journal of molecular sciences · 2020Pooled it
- Monocyte Dll4 Contributes to Systemic Inflammation in People Living With HIV.Circulation research · 2026Article
- Effect of reticulocalbin 3 on monocyte-to-macrophage differentiation in sepsis through modulating autophagy.Chinese medical journal · 2026Article
- Profile of tRNA-derived short non-coding RNAs during monocyte differentiation and their role in macrophage survival.RNA biology · 2025Article
- Myeloid AMPK signaling restricts fibrosis but is not required for metformin improvements during CDAHFD-induced NASH in mice.Journal of lipid research · 2024Article
- Transcriptomic Context ofBiomedicines · 2023Article
- PLAUR splicing pattern in hereditary angioedema patients' monocytes and macrophages.Molecular biology reports · 2023Article
- Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA.PloS one · 2023Article
- Temporal Analysis Reveals the Transient Differential Expression of Transcription Factors That Underlie the Trans-Differentiation of Human Monocytes to Macrophages.International journal of molecular sciences · 2022Article
- Macrophage scavenger receptor 1 mediates lipid-induced inflammation in non-alcoholic fatty liver disease.Journal of hepatology · 2022Article
- Induced Pluripotent Stem Cell-Derived Monocytes/Macrophages in Autoinflammatory Diseases.Frontiers in immunology · 2022Review
- Single-cell transcriptome analysis reveals heterogeneity and convergence of the tumor microenvironment in colorectal cancer.Frontiers in immunology · 2022Article
- Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo.International journal of molecular sciences · 2021Article
- PAQR11 modulates monocyte-to-macrophage differentiation and pathogenesis of rheumatoid arthritis.Immunology · 2021Article
- Myeloid deletion and therapeutic activation of AMPK do not alter atherosclerosis in male or female mice.Journal of lipid research · 2020Article
- Article
- Regeneration associated transcriptional signature of retinal microglia and macrophages.Scientific reports · 2019Article
- Rapid flow cytometry-based assay for the evaluation of γδ T cell-mediated cytotoxicity.Molecular medicine reports · 2018Article
- Angiogenesis and evading immune destruction are the main related transcriptomic characteristics to the invasive process of oral tongue cancer.Scientific reports · 2018Article
- Salicylate improves macrophage cholesterol homeostasis via activation of Ampk.Journal of lipid research · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Monocytes can be differentiated into macrophages in vivo and these cells play an important role in innate and adaptive immune responses. To reveal the global gene transcription change that occurs during monocyte to macrophage differentiation, we performed genome-wide RNA sequencing and analyses in human primary monocytes and monocyte-derived macrophages. We show that 1208 genes (with >twofold differences) were differentially expressed in macrophages compared with monocytes, including 800 upregulated and 408 downregulated genes. Gene ontology, pathway, and protein-protein interaction analyses indicated that the upregulated genes were related to macrophage functions in phagocytosis, metabolic processes, and cell cycle. The majority of downregulated genes comprised genes involved in the inflammatory response and locomotion. Genes encoding transcription regulatory factors, such as FOXO1, RUNX3, NF-κB1, and C/EBP δ, were highly expressed in monocytes and appeared to function in significant transcriptional repression, resulting in slight metabolic activity. Our transcriptome comparison between human monocytes and monocyte-derived macrophages using RNA sequencing revealed novel molecules and pathways associated with the differentiation process. These molecules and pathways may represent candidate targets involved in the pathophysiology of these important immune cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.