ArticleMicroscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada2013
Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling.
Article in Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- The 50-nm Free Vesicles Visible inCurrent issues in molecular biology · 2025Article
- Mechanistic divergences of endocytic clathrin-coated vesicle formation in mammals, yeasts and plants.Journal of cell science · 2024Review
- Nitrogen limitation-induced adaptive response and lipogenesis in the Antarctic yeastFrontiers in microbiology · 2024Article
- Load adaptation by endocytic actin networks.Molecular biology of the cell · 2022Article
- A model of actin-driven endocytosis explains differences of endocytic motility in budding and fission yeast.Molecular biology of the cell · 2022Article
- ER-phagy requires the assembly of actin at sites of contact between the cortical ER and endocytic pits.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Plasma Membrane MCC/Eisosome Domains Promote Stress Resistance in Fungi.Microbiology and molecular biology reviews : MMBR · 2020Review
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- Role of membrane compartment occupied by Can1 (MCC) and eisosome subdomains in plant pathogenicity of the necrotrophic fungus Alternaria brassicicola.BMC microbiology · 2019Article
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- TheJournal of cell science · 2019Article
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- The role of traction in membrane curvature generation.Molecular biology of the cell · 2018Article
- High-speed superresolution imaging of the proteins in fission yeast clathrin-mediated endocytic actin patches.Molecular biology of the cell · 2018Article
- MCC/Eisosomes Regulate Cell Wall Synthesis and Stress Responses in Fungi.Journal of fungi (Basel, Switzerland) · 2017Review
- Proteomic and genetic analysis of the response of S. cerevisiae to soluble copper leads to improvement of the antimicrobial function of cellulosic copper nanoparticles.Metallomics : integrated biometal science · 2017Article
- Plasma membrane organization promotes virulence of the human fungal pathogen Candida albicans.Journal of microbiology (Seoul, Korea) · 2016Review
- Developmental Coordination of Gamete Differentiation with Programmed Cell Death in Sporulating Yeast.Eukaryotic cell · 2015Article
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2 authors.
Funding
Abstract
Defining the ultrastructure of endocytic sites and localization of endocytic proteins in Saccharomyces cerevisiae by immunoelectron microscopy is central in understanding the mechanisms of membrane deformation and scission during endocytosis. We show that an improved sample preparation protocol based on high-pressure freezing, freeze substitution, and low-temperature embedding allows us to maintain the cellular fine structure and to immunolabel green fluorescent protein-tagged endocytic proteins or actin in the same sections. Using this technique we analyzed the stepwise deformation of endocytic membranes and immunolocalized the endocytic proteins Abp1p, Sla1p, Rvs167p, and actin, and were able to draw a clear ultrastructural distinction between endocytic sites and eisosomes by immunolocalizing Pil1p. In addition to defining the geometry and the fine structure of budding yeast endocytic sites, we observed associated actin filaments forming a cage-like meshwork around the endocytic membrane.
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