ArticleAnalytical biochemistry2013
Recorded scan times can limit the accuracy of sedimentation coefficients in analytical ultracentrifugation.
Article in Analytical biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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69 citing papers in PubMed.
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- Mimicking Protein Kinase C Phosphorylation Inhibits Arc/Arg3.1 Palmitoylation and Its Interaction with Nucleic Acids.International journal of molecular sciences · 2024Article
- An automated interface for sedimentation velocity analysis in SEDFIT.PLoS computational biology · 2023Article
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- An automated interface for sedimentation velocity analysis in SEDFIT.bioRxiv : the preprint server for biology · 2023Article
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- Bivalent recognition of fatty acyl-CoA by a human integral membrane palmitoyltransferase.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Controlling the conformational stability of coiled-coil peptides with a single stereogenic center of a peripheral β-amino acid residue.RSC advances · 2022Article
- HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization.Cell host & microbe · 2021Article
- Natural Mutations Affect Structure and Function of gC1q Domain of Otolin-1.International journal of molecular sciences · 2021Article
9 more citing papers are in PubMed but not listed here.
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6 authors.
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Abstract
We report systematic and large inaccuracies in the recorded elapsed time in data files from the analytical ultracentrifuge, leading to overestimates of the sedimentation coefficients of up to 10%. This far exceeds previously considered factors contributing to the uncertainty in this parameter and has significant ramifications for derived parameters such as hydrodynamic shape and molar mass estimates. The source of this error is currently unknown, but we found it to be quantitatively consistent across different instruments, increasing with rotor speed. Furthermore, its occurrence appears to correlate with the use of the latest data acquisition software from the manufacturer, in use in some of our laboratories for nearly 2 years. Many of the recently published sedimentation coefficients may need to be reexamined. The problem can be easily recognized by comparing the file timestamps provided by the operating system with the elapsed scan times recorded within the data files. Therefore, we implemented a routine in SEDFIT that can automatically examine the data files, alert the user to significant discrepancies, and correct the scan times accordingly. This eliminates errors in the recorded scan times.
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