ArticlePloS one2013
Dynamic light scattering study of inhibition of nucleation and growth of hydroxyapatite crystals by osteopontin.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Elastin calcification during the initial stage of vascular calcification in kl/kl mice is suppressed by the calcimimetic upacicalcet.Scientific reports · 2026Article
- Inflammation-induced osteogenic signaling promotes calcium phosphate crystal formation in kidneys via MAPK, NF-κB, and smad pathways.Frontiers in cell and developmental biology · 2026Article
- Evidence for a possible quantum effect on the formation of lithium-doped amorphous calcium phosphate from solution.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Structure of biomimetic casein micelles: Critical tests of the hydrophobic colloid and multivalent-binding models using recombinant deuterated and phosphorylated β-casein.Journal of structural biology: X · 2024Article
- Prenatal and Neonatal Bone Health: Updated Review on Early Identification of Newborns at High Risk for Osteopenia.Nutrients · 2023Review
- Matrix Vesicle-Mediated Mineralization and Osteocytic Regulation of Bone Mineralization.International journal of molecular sciences · 2022Review
- Enzymatic Approach in Calcium Phosphate Biomineralization: A Contribution to Reconcile the Physicochemical with the Physiological View.International journal of molecular sciences · 2021Review
- Binding Mode Characterization of Osteopontin on Hydroxyapatite by Solution NMR Spectroscopy.Chembiochem : a European journal of chemical biology · 2021Article
- Hyperphosphorylation of Human Osteopontin and Its Impact on Structural Dynamics and Molecular Recognition.Biochemistry · 2021Article
- Osteopontin regulates biomimetic calcium phosphate crystallization from disordered mineral layers covering apatite crystallites.Scientific reports · 2020Article
- FGF adsorbed mesoporous bioactive glass with larger pores in enhancing bone tissue engineering.Journal of materials science. Materials in medicine · 2019Article
- The effect of polyaspartate chain length on mediating biomimetic remineralization of collagenous tissues.Journal of the Royal Society, Interface · 2018Article
- Targeted Nanoparticle Binding to Hydroxyapatite in a High Serum Environment for Early Detection of Heart Disease.ACS applied nano materials · 2018Article
- Phenotypic Spectrum in Osteogenesis Imperfecta Due to Mutations in TMEM38B: Unraveling a Complex Cellular Defect.The Journal of clinical endocrinology and metabolism · 2017Article
- Structural studies of hydrated samples of amorphous calcium phosphate and phosphoprotein nanoclusters.European biophysics journal : EBJ · 2016Article
- Supraphysiological Levels of Quercetin Glycosides are Required to Alter Mineralization in Saos2 Cells.International journal of environmental research and public health · 2016Article
- Simultaneous bactericidal and osteogenic effect of nanoparticulate calcium phosphate powders loaded with clindamycin on osteoblasts infected with Staphylococcus aureus.Materials science & engineering. C, Materials for biological applications · 2014Article
- Biomimetic remineralization of dentin.Dental materials : official publication of the Academy of Dental Materials · 2014Article
- Peptides of Matrix Gla protein inhibit nucleation and growth of hydroxyapatite and calcium oxalate monohydrate crystals.PloS one · 2013Article
- Intrinsically disordered proteins and biomineralization.Matrix biology : journal of the International Society for Matrix BiologyReview
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
We study the effect of isoforms of osteopontin (OPN) on the nucleation and growth of crystals from a supersaturated solution of calcium and phosphate ions. Dynamic light scattering is used to monitor the size of the precipitating particles and to provide information about their concentration. At the ion concentrations studied, immediate precipitation was observed in control experiments with no osteopontin in the solution, and the size of the precipitating particles increased steadily with time. The precipitate was identified as hydroxyapatite by X-ray diffraction. Addition of native osteopontin (nOPN) extracted from rat bone caused a delay in the onset of precipitation and reduced the number of particles that formed, but the few particles that did form grew to a larger size than in the absence of the protein. Recombinant osteopontin (rOPN), which lacks phosphorylation, caused no delay in initial calcium phosphate precipitation but severely slowed crystal growth, suggesting that rOPN inhibits growth but not nucleation. rOPN treated with protein kinase CK2 to phosphorylate the molecule (p-rOPN) produced an effect similar to that of nOPN, but at higher protein concentrations and to a lesser extent. These results suggest that phosphorylations are critical to OPN's ability to inhibit nucleation, whereas the growth of the hydroxyapatite crystals is effectively controlled by the highly acidic OPN polypeptide. This work also demonstrates that dynamic light scattering can be a powerful tool for delineating the mechanism of protein modulation of mineral formation.
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Registered trials
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