ArticlePloS one2013
Air-stimulated ATP release from keratinocytes occurs through connexin hemichannels.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 60 citations in OpenAlex.
- A transitional desmosome/tonofibril network may relay mechanical strain to epidermal nerve terminals with high fidelity and sensitivity in the Cuban crocodile (Frontiers in cell and developmental biology · 2026Article
- Article
- Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli in Drosophila larvae.PLoS genetics · 2024Article
- Interaction of human keratinocytes and nerve fiber terminals at the neuro-cutaneous unit.eLife · 2024Article
- Mechanical stimulation of human hair follicle outer root sheath cultures activates adjacent sensory neurons.Science advances · 2023Article
- Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli inbioRxiv : the preprint server for biology · 2023Article
- Article
- Calcium Signaling in the Photodamaged Skin: In Vivo Experiments and Mathematical Modeling.Function (Oxford, England) · 2022Article
- Mechanisms of ATP release in pain: role of pannexin and connexin channels.Purinergic signalling · 2021Review
- Cutaneous pain in disorders affecting peripheral nerves.Neuroscience letters · 2021Review
- Analysis of Cytokines and ATP in Plucked Hair Follicles.Bulletin of experimental biology and medicine · 2021Article
- Keratinocyte Biomarkers Distinguish Painful Diabetic Peripheral Neuropathy Patients and Correlate With Topical Lidocaine Responsiveness.Frontiers in pain research (Lausanne, Switzerland) · 2021Article
- A potent antagonist antibody targeting connexin hemichannels alleviates Clouston syndrome symptoms in mutant mice.EBioMedicine · 2020Article
- Lifting the veil on the keratinocyte contribution to cutaneous nociception.Protein & cell · 2020Review
- A Role for Neutral Sphingomyelinase in Wound Healing Induced by Keratinocyte Proliferation upon 1International journal of molecular sciences · 2019Article
- Expression and function of the ectopic olfactory receptor OR10G7 in patients with atopic dermatitis.The Journal of allergy and clinical immunology · 2019Article
- Role of Keratinocytes in Sensitive Skin.Frontiers in medicine · 2019Review
- Quantitative Top-Down Mass Spectrometry Identifies Proteoforms Differentially Released during Mechanical Stimulation of Mouse Skin.Journal of proteome research · 2018Article
- Article
- Cell culture: complications due to mechanical release of ATP and activation of purinoceptors.Cell and tissue research · 2017Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Cutaneous ATP release plays an important role in both epidermal stratification and chronic pain, but little is known about ATP release mechanisms in keratinocytes that comprise the epidermis. In this study, we analyzed ATP release from cultured human neonatal keratinocytes briefly exposed to air, a process previously demonstrated to trigger ATP release from these cells. We show that exposing keratinocytes to air by removing media for 15 seconds causes a robust, long-lasting ATP release. This air-stimulated ATP release was increased in calcium differentiated cultures which showed a corresponding increase in connexin 43 mRNA, a major component of keratinocyte hemichannels. The known connexin hemichannel inhibitors 1-octanol and carbenoxolone both significantly reduced air-stimulated ATP release, as did two drugs traditionally used as ABC transporter inhibitors (glibenclamide and verapamil). These same 4 inhibitors also prevented an increase in the uptake of a connexin permeable dye induced by air exposure, confirming that connexin hemichannels are open during air-stimulated ATP release. In contrast, activity of the MDR1 ABC transporter was reduced by air exposure and the drugs that inhibited air-stimulated ATP release had differential effects on this transporter. These results indicate that air exposure elicits non-vesicular release of ATP from keratinocytes through connexin hemichannels and that drugs used to target connexin hemichannels and ABC transporters may cross-inhibit. Connexins represent a novel, peripheral target for the treatment of chronic pain and dermatological disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.