ArticlePloS one2013
Epithelial cells as active player in fibrosis: findings from an in vitro model.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- AI-enabled label free monitoring of TGFβ1-induced remodeling in iPSC-derived alveolar organoids.Materials today. Bio · 2026Article
- Emerging Perspectives in Anti-aging Treatments Using Anti-Fibrotic Strategies.Aging and disease · 2025Review
- Kidney Fibrosis In Vitro and In Vivo Models: Path Toward Physiologically Relevant Humanized Models.Advanced healthcare materials · 2025Review
- 3D Humanized Bioprinted Tubulointerstitium Model to Emulate Renal Fibrosis In Vitro.Advanced healthcare materials · 2024Article
- Application of microfluidic chips in the simulation of the urinary system microenvironment.Materials today. Bio · 2023Review
- NF-κB drives epithelial-mesenchymal mechanisms of lung fibrosis in a translational lung cell model.JCI insight · 2023Article
- Lysophosphatidic Acid Is a Proinflammatory Stimulus of Renal Tubular Epithelial Cells.International journal of molecular sciences · 2022Article
- Role of extracellular matrix components and structure in new renal modelsFrontiers in physiology · 2022Review
- Vegfa promoter gene hypermethylation at HIF1α binding site is an early contributor to CKD progression after renal ischemia.Scientific reports · 2021Article
- Ischaemia reperfusion injury: mechanisms of progression to chronic graft dysfunction.Pediatric nephrology (Berlin, Germany) · 2019Review
- Gcm1 is involved in cell proliferation and fibrosis during kidney regeneration after ischemia-reperfusion injury.Scientific reports · 2019Article
- Renal Damaging Effect Elicited by Bicalutamide Therapy Uncovered Multiple Action Mechanisms As Evidenced by the Cell Model.Scientific reports · 2019Article
- Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV Integrins.Nephron · 2019Article
- Selective pharmacological inhibition of DDR1 prevents experimentally-induced glomerulonephritis in prevention and therapeutic regime.Journal of translational medicine · 2018Article
- Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor β1.BMC veterinary research · 2018Article
- Monitoring and manipulating cellular crosstalk during kidney fibrosis inside a 3D in vitro co-culture.Scientific reports · 2017Article
- An immunofluorescence assay for extracellular matrix components highlights the role of epithelial cells in producing a stable, fibrillar extracellular matrix.Biology open · 2017Article
- 3D Proximal Tubule Tissues Recapitulate Key Aspects of Renal Physiology to Enable Nephrotoxicity Testing.Frontiers in physiology · 2017Article
- Mimicking the Kidney: A Key Role in Organ-on-Chip Development.Micromachines · 2016Review
- The proximal tubule is the primary target of injury and progression of kidney disease: role of the glomerulotubular junction.American journal of physiology. Renal physiology · 2016Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kidney fibrosis, a scarring of the tubulo-interstitial space, is due to activation of interstitial myofibroblasts recruited locally or systemically with consecutive extracellular matrix deposition. Newly published clinical studies correlating acute kidney injury (AKI) to chronic kidney disease (CKD) challenge this pathological concept putting tubular epithelial cells into the spotlight. In this work we investigated the role of epithelial cells in fibrosis using a simple controlled in vitro system. An epithelial/mesenchymal 3D cell culture model composed of human proximal renal tubular cells and fibroblasts was challenged with toxic doses of Cisplatin, thus injuring epithelial cells. RT-PCR for classical fibrotic markers was performed on fibroblasts to assess their modulation toward an activated myofibroblast phenotype in presence or absence of that stimulus. Epithelial cell lesion triggered a phenotypical modulation of fibroblasts toward activated myofibroblasts as assessed by main fibrotic marker analysis. Uninjured 3D cell culture as well as fibroblasts alone treated with toxic stimulus in the absence of epithelial cells were used as control. Our results, with the caveats due to the limited, but highly controllable and reproducible in vitro approach, suggest that epithelial cells can control and regulate fibroblast phenotype. Therefore they emerge as relevant target cells for the development of new preventive anti-fibrotic therapeutic approaches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.