ArticlePloS one2013
Characterization of Osterix protein stability and physiological role in osteoblast differentiation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
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- Moxibustion ameliorates abnormal subchondral bone remodeling by promoting ACSL1-mediated autophagy to degrade NLRP3 in osteoarthritis.Chinese medicine · 2025Article
- MiR-224-5p inhibits osteoblast differentiation and impairs bone formation by targeting Runx2 and Sp7.Cytotechnology · 2023Article
- Deubiquitinase USP17 Regulates Osteoblast Differentiation by Increasing Osterix Protein Stability.International journal of molecular sciences · 2023Article
- Low Magnesium Concentration Enforces Bone Calcium Deposition Irrespective of 1,25-Dihydroxyvitamin DInternational journal of molecular sciences · 2023Article
- Enamel matrix derivative expedites osteogenic differentiation of BMSCs via Wnt/β-catenin pathway in high glucose microenvironment.Journal of bone and mineral metabolism · 2022Article
- The Effects of Polyphenol, Tannic Acid, or Tannic Acid in Combination with Pamidronate on Human Osteoblast Cell Line Metabolism.Molecules (Basel, Switzerland) · 2022Article
- BGP-15 Inhibits Hyperglycemia-Aggravated VSMC Calcification Induced by High Phosphate.International journal of molecular sciences · 2021Article
- E3 Ubiquitin Ligase-Mediated Regulation of Osteoblast Differentiation and Bone Formation.Frontiers in cell and developmental biology · 2021Review
- ASF1B promotes cervical cancer progression through stabilization of CDK9.Cell death & disease · 2020Article
- Recent Advances of Osterix Transcription Factor in Osteoblast Differentiation and Bone Formation.Frontiers in cell and developmental biology · 2020Review
- A comparative in vitro study of the osteogenic and adipogenic potential of human dental pulp stem cells, gingival fibroblasts and foreskin fibroblasts.Scientific reports · 2019Article
- SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7/Osterix.Nature communications · 2018Article
- Article
- Melatonin promotes osteoblast differentiation by regulating Osterix protein stability and expression.Scientific reports · 2017Article
- Alteration of Wnt5a expression and of the non-canonical Wnt/PCP and Wnt/PKC-Ca2+ pathways in human osteoarthritis osteoblasts.PloS one · 2017Article
- Osterix acetylation at K307 and K312 enhances its transcriptional activity and is required for osteoblast differentiation.Oncotarget · 2016Article
- TGF-β Signaling Regulates Cementum Formation through Osterix Expression.Scientific reports · 2016Article
- Identification of a novel selective agonist of PPARγ with no promotion of adipogenesis and less inhibition of osteoblastogenesis.Scientific reports · 2015Article
- Nanostructured platforms for the sustained and local delivery of antibiotics in the treatment of osteomyelitis.Critical reviews in therapeutic drug carrier systems · 2015Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Osterix (Osx/SP7) is a C2H2 zinc finger-containing transcription factor of the SP gene family. Osx knockout mice indicate that the gene plays an essential role in osteoblast differentiation and bone formation. However, the mechanisms involved in the regulation of Osx are still poorly understood. Here, we report a novel post-translational mechanism for the regulation of Osx in mammalian cells. We found that the stability of endogenous and exogenous Osx reduced after cycloheximide treatment. In cells treated with the proteasome inhibitors MG-132 or lactacystin, both endogenous and exogenous Osx protein expression increased in a time-dependent manner. Co-immunoprecipitation (Co-IP) assays showed that both endogenous and exogenous Osx were ubiquitinated. Six lysine residues of Osx were identified as candidate ubiquitination sites by construction of point mutant plasmids and luciferase reporter assays. Furthermore, we confirmed that K58 and K230 are the ubiquitination sites of Osx by Co-IP assays and protein stability assays. Moreover, the Osx K58R and K230R mutations promoted the expression of osteoblast differentiation markers (alkaline phosphatase, collagen I and osteocalcin) and enhanced osteogenic differentiation in C2C12 cells. Taken together, our data indicate that Osx is an unstable protein, and that the ubiquitin-proteasome pathway is involved in the regulation of Osx and thereby regulates osteoblast differentiation.
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