ArticlePloS one2013
MALDI-TOF MS distinctly differentiates nontypable Haemophilus influenzae from Haemophilus haemolyticus.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Patients with Chronic Obstructive Pulmonary Disease harbour a variation of Haemophilus species.Scientific reports · 2018Trial
- A one-pot CRISPR/Cas12b assay for extraction-free and visual detection of Haemophilus influenzae.BMC microbiology · 2026Article
- High-resolution differentiation and identification of the clinically relevant pathogensMicrobiology spectrum · 2026Article
- Amplicon-based sequencing as a diagnostic tool for severe pneumonia in the ICU.Scientific reports · 2026Article
- Metagenomic sequencing reveals swine lung microbial communities and metagenome-assembled genomes associated with lung lesions-a pilot study.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Clinical and Bacteriologic Analysis of Nontypeable Haemophilus influenzae Strains Isolated from Children with Invasive Diseases in Japan from 2008 to 2015.Journal of clinical microbiology · 2018Article
- Recent Advances and Ongoing Challenges in the Diagnosis of Microbial Infections by MALDI-TOF Mass Spectrometry.Frontiers in microbiology · 2018Article
- Rapid Differentiation of Haemophilus influenzae and Haemophilus haemolyticus by Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry with ClinProTools Mass Spectrum Analysis.Journal of clinical microbiology · 2017Article
- Comparative Genomic Analysis of Haemophilus haemolyticus and Nontypeable Haemophilus influenzae and a New Testing Scheme for Their Discrimination.Journal of clinical microbiology · 2016Article
- The Lung Microbiome, Immunity, and the Pathogenesis of Chronic Lung Disease.Journal of immunology (Baltimore, Md. : 1950) · 2016Review
- Comparison of Established Diagnostic Methodologies and a Novel Bacterial smpB Real-Time PCR Assay for Specific Detection of Haemophilus influenzae Isolates Associated with Respiratory Tract Infections.Journal of clinical microbiology · 2015Article
- Republished: Respiratory microbiota: addressing clinical questions, informing clinical practice.Postgraduate medical journal · 2015Review
- Difficult identification of Haemophilus influenzae, a typical cause of upper respiratory tract infections, in the microbiological diagnostic routine.European journal of microbiology & immunology · 2015Review
- Review
- A review of the role ofPneumonia (Nathan Qld.) · 2015Review
- Article
- Classification, identification, and clinical significance of Haemophilus and Aggregatibacter species with host specificity for humans.Clinical microbiology reviews · 2014Review
- Lower airway colonization and inflammatory response in COPD: a focus on Haemophilus influenzae.International journal of chronic obstructive pulmonary disease · 2014Review
- Molecular tools for differentiation of non-typeable Haemophilus influenzae from Haemophilus haemolyticus.Frontiers in microbiology · 2014Review
- The construction and evaluation of reference spectra for the identification of human pathogenic microorganisms by MALDI-TOF MS.PloS one · 2014Article
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Authors and funding
10 authors.
Funding
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Abstract
Nontypable Haemophilus influenzae (NTHi) and Haemophilus haemolyticus exhibit different pathogenicities, but to date, there remains no definitive and reliable strategy for differentiating these strains. In this study, we evaluated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a potential method for differentiating NTHi and H. haemolyticus. The phylogenetic analysis of concatenated 16S rRNA and recombinase A (recA) gene sequences, outer membrane protein P6 gene sequencing and single-gene PCR were used as reference methods. The original reference database (ORD, provided with the Biotyper software) and new reference database (NRD, extended with Chinese strains) were compared for the evaluation of MALDI-TOF MS. Through a search of the ORD, 76.9% of the NTHi (40/52) and none of the H. haemolyticus (0/20) strains were identified at the species level. However, all NTHi and H. haemolyticus strains used for identification were accurately recognized at the species level when searching the NRD. From the dendrogram clustering of the main spectra projections, the Chinese and foreign H. influenzae reference strains were categorized into two distinct groups, and H. influenzae and H. haemolyticus were also separated into two categories. Compared to the existing methods, MALDI-TOF MS has the advantage of integrating high throughput, accuracy and speed. In conclusion, MALDI-TOF MS is an excellent method for differentiating NTHi and H. haemolyticus. This method can be recommended for use in appropriately equipped laboratories.
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