ArticleGenetics2013
The nuclear argonaute NRDE-3 contributes to transitive RNAi in Caenorhabditis elegans.
Article in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Distinct regulatory mechanisms by the nuclear Argonautes HRDE-1 and NRDE-3 in the soma of Caenorhabditis elegans.G3 (Bethesda, Md.) · 2025Article
- Peri-centrosomal localization of small interfering RNAs in C. elegans.Science China. Life sciences · 2025Article
- A Small-RNA-Mediated Feedback Loop Maintains Proper Levels of 22G-RNAs in C. elegans.Cell reports · 2020Article
- A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi.Molecular cell · 2020Article
- Multigenerational Regulation of theAnnual review of genetics · 2019Review
- Article
- Intergenerational Transmission of Gene Regulatory Information in Caenorhabditis elegans.Trends in genetics : TIG · 2018Review
- Early Developmental Exposure to dsRNA Is Critical for Initiating Efficient Nuclear RNAi in C. elegans.Cell reports · 2017Article
- Stable Heritable Germline Silencing Directs Somatic Silencing at an Endogenous Locus.Molecular cell · 2017Article
- Antiviral RNA Interference against Orsay Virus Is neither Systemic nor Transgenerational in Caenorhabditis elegans.Journal of virology · 2015Article
- Movement of regulatory RNA between animal cells.Genesis (New York, N.Y. : 2000) · 2015Review
- Lack of pairing during meiosis triggers multigenerational transgene silencing in Caenorhabditis elegans.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Tertiary siRNAs mediate paramutation in C. elegans.PLoS genetics · 2015Article
- Small RNAs break out: the molecular cell biology of mobile small RNAs.Nature reviews. Molecular cell biology · 2014Review
- Nuclear RNAi contributes to the silencing of off-target genes and repetitive sequences in Caenorhabditis elegans.Genetics · 2014Article
- Assays for direct and indirect effects of C. elegans endo-siRNAs.Methods in molecular biology (Clifton, N.J.) · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Caenorhabditis elegans nuclear RNA interference defective (Nrde) mutants were identified by their inability to silence polycistronic transcripts in enhanced RNAi (Eri) mutant backgrounds. Here, we report additional nrde-3-dependent RNAi phenomena that extend the mechanisms, roles, and functions of nuclear RNAi. We show that nrde-3 mutants are broadly RNAi deficient and that overexpressing NRDE-3 enhances RNAi. Consistent with NRDE-3 being a dose-dependent limiting resource for effective RNAi, we find that NRDE-3 is required for eri-dependent enhanced RNAi phenotypes, although only for a subset of target genes. We then identify pgl-1 as an additional limiting RNAi resource important for eri-dependent silencing of a nonoverlapping subset of target genes, so that an nrde-3; pgl-1; eri-1 triple mutant fails to show enhanced RNAi for any tested gene. These results suggest that nrde-3 and pgl-1 define separate and independent limiting RNAi resource pathways. Limiting RNAi resources are proposed to primarily act via endogenous RNA silencing pathways. Consistent with this, we find that nrde-3 mutants misexpress genes regulated by endogenous siRNAs and incompletely silence repetitive transgene arrays. Finally, we find that nrde-3 contributes to transitive RNAi, whereby amplified silencing triggers act in trans to silence sequence-similar genes. Because nrde-dependent silencing is thought to act in cis to limit the production of primary transcripts, this result reveals an unexpected role for nuclear processes in RNAi silencing.
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