ArticlePlant physiology2013
Correlative imaging of fluorescent proteins in resin-embedded plant material.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 81 citations in OpenAlex.
- Selective degradation processes in plants are not limited to autophagy.Plant cell reports · 2026Review
- Characterization of Viruses in Phloem by Correlative X-Ray Microtomography (μCT)-Volume Electron Microscopy (vEM) Imaging.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Transmission Electron Microscopy of the Phloem.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- In-section Click-iT detection and super-resolution CLEM analysis of nucleolar ultrastructure and replication in plants.Nature communications · 2024Article
- Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The rapid-tome, a 3D-printed microtome, and an updated hand-sectioning method for high-quality plant sectioning.Plant methods · 2023Article
- Targeting Ultrastructural Events at the Graft Interface ofBio-protocol · 2023Article
- Short Peptides Induce Development of Root HairPlants (Basel, Switzerland) · 2022Article
- A correlative light electron microscopy approach reveals plasmodesmata ultrastructure at the graft interface.Plant physiology · 2022Article
- One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM.Frontiers in cell and developmental biology · 2022Review
- Spatially resolved correlative microscopy and microbial identification reveal dynamic depth- and mineral-dependent anabolic activity in salt marsh sediment.Environmental microbiology · 2021Article
- A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography.BMC biology · 2021Article
- Reactive oxygen FIB spin milling enables correlative workflow for 3D super-resolution light microscopy and serial FIB/SEM of cultured cells.Scientific reports · 2021Article
- Identification and Characterization of Highly Fluorescent Pigment Cells in Embryos of the Arabian Killifish (iScience · 2020Article
- Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish.Biology open · 2019Article
- Nodulin Intrinsic Protein 7;1 Is a Tapetal Boric Acid Channel Involved in Pollen Cell Wall Formation.Plant physiology · 2018Article
- Automated detection of fluorescent cells in in-resin fluorescence sections for integrated light and electron microscopy.Journal of microscopy · 2018Article
- Tissue Distribution and Visualization of Internalized Human Norovirus in Leafy Greens.Applied and environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Fluorescent proteins (FPs) were developed for live-cell imaging and have revolutionized cell biology. However, not all plant tissues are accessible to live imaging using confocal microscopy, necessitating alternative approaches for protein localization. An example is the phloem, a tissue embedded deep within plant organs and sensitive to damage. To facilitate accurate localization of FPs within recalcitrant tissues, we developed a simple method for retaining FPs after resin embedding. This method is based on low-temperature fixation and dehydration, followed by embedding in London Resin White, and avoids the need for cryosections. We show that a palette of FPs can be localized in plant tissues while retaining good structural cell preservation, and that the polymerized block face can be counterstained with cell wall probes. Using this method we have been able to image green fluorescent protein-labeled plasmodesmata to a depth of more than 40 μm beneath the resin surface. Using correlative light and electron microscopy of the phloem, we were able to locate the same FP-labeled sieve elements in semithin and ultrathin sections. Sections were amenable to antibody labeling, and allowed a combination of confocal and superresolution imaging (three-dimensional-structured illumination microscopy) on the same cells. These correlative imaging methods should find several uses in plant cell biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.