ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2013
A copper sulfate and hydroxylysine treatment regimen for enhancing collagen cross-linking and biomechanical properties in engineered neocartilage.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 70 citations in OpenAlex.
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- Challenges and recent advances in engineering the osteochondral interface.Current opinion in biomedical engineering · 2024Article
- Engineered Proteins and Materials Utilizing Residue-Specific Noncanonical Amino Acid Incorporation.Chemical reviews · 2024Review
- Copper metabolism in osteoarthritis and its relation to oxidative stress and ferroptosis in chondrocytes.Frontiers in molecular biosciences · 2024Review
- Carnosine, Zinc and Copper: A Menage a Trois in Bone and Cartilage Protection.International journal of molecular sciences · 2023Review
- Exploring the Interplay between Polyphenols and Lysyl Oxidase Enzymes for Maintaining Extracellular Matrix Homeostasis.International journal of molecular sciences · 2023Review
- Temporal application of lysyl oxidase during hierarchical collagen fiber formation differentially effects tissue mechanics.Acta biomaterialia · 2023Article
- Silk-based hydrogel incorporated with metal-organic framework nanozymes for enhanced osteochondral regeneration.Bioactive materials · 2023Article
- Micronutrient optimization for tissue engineered articular cartilage production of type II collagen.Frontiers in bioengineering and biotechnology · 2023Article
- Enhanced osteoarthritis therapy by nanoengineered mesenchymal stem cells using biomimetic CuS nanoparticles loaded with plasmid DNA encoding TGF-β1.Bioactive materials · 2023Article
- Ion modulatory treatments toward functional self-assembled neocartilage.Acta biomaterialia · 2022Article
- Non-destructive, continuous monitoring of biochemical, mechanical, and structural maturation in engineered tissue.Scientific reports · 2022Article
- Regulators of collagen crosslinking in developing and adult tendons.European cells & materials · 2022Review
- The functionality and translatability of neocartilage constructs are improved with the combination of fluid-induced shear stress and bioactive factors.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Tissue Engineering of Canine Cartilage from Surgically Debrided Osteochondritis Dissecans Fragments.Annals of biomedical engineering · 2022Article
- Tuning the Properties of PNIPAm-Based Hydrogel Scaffolds for Cartilage Tissue Engineering.Polymers · 2021Review
- Copper-based biomaterials for bone and cartilage tissue engineering.Journal of orthopaedic translation · 2021Review
- Peroxiredoxins-The Underrated Actors during Virus-Induced Oxidative Stress.Antioxidants (Basel, Switzerland) · 2021Review
- Engineering self-assembled neomenisci through combination of matrix augmentation and directional remodeling.Acta biomaterialia · 2020Article
- Molecular Insights Into Lysyl Oxidases in Cartilage Regeneration and Rejuvenation.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The objective of this study was to improve the biomechanical properties of engineered neotissues through promoting the development of collagen cross-links. It was hypothesized that supplementing medium with copper sulfate and the amino acid hydroxylysine would enhance the activity of lysyl oxidase enzyme to form collagen cross-links, increasing the strength and integrity of the neotissue. Neocartilage constructs were generated using a scaffoldless, self-assembling process and treated with copper sulfate and hydroxylysine, either alone or in combination, following a 2-factor, full-factorial study design. Following a 6-wk culture period, the biomechanical and biochemical properties of the constructs were measured. Results found copper sulfate to significantly increase pyridinoline (PYR) cross-links in all copper sulfate-containing groups over controls. When copper sulfate and hydroxylysine were combined, the result was synergistic, with a 10-fold increase in PYR content over controls. This increase in PYR cross-links manifested in a 3.3-fold significant increase in the tensile properties of the copper sulfate + hydroxylysine group. In addition, an 123% increase over control values was detected in the copper sulfate group in terms of the aggregate modulus. These data elucidate the role of copper sulfate and hydroxylysine toward improving the biomechanical properties of neotissues through collagen cross-linking enhancement.
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Registered trials
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