ArticleEukaryotic cell2013
Detection of fungal DNA in human body fluids and tissues during a multistate outbreak of fungal meningitis and other infections.
Article in Eukaryotic cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 80 citations in OpenAlex.
- Fungal Meningitis in US Patients Who Received Epidural Anesthesia in Matamoros, Mexico.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2026Article
- Pseudo-Outbreak of Purpureocillium lilacinum Skin Infections at a Dermatology Clinic - Washington, 2024.MMWR. Morbidity and mortality weekly report · 2026Article
- Evaluation of metagenomic next-generation sequencing (mNGS) combined with quantitative PCR: cutting-edge methods for rapid diagnosis of non-invasive fungal rhinosinusitis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Effects of combined application of pig manure composts prepared using different fermentation methods with chemical fertilizer on winter wheat yield, light-thermal physiology, and soil biological characteristics.Frontiers in plant science · 2025Article
- Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections.Clinical microbiology reviews · 2024Review
- Outcomes in solid organ transplant recipients receiving organs from a donor with Fusarium solani species complex meningitis.Transplant infectious disease : an official journal of the Transplantation Society · 2024Article
- Outbreaks of Fungal Infections in Hospitals: Epidemiology, Detection, and Management.Journal of fungi (Basel, Switzerland) · 2023Review
- Soil Microbial Network Complexity Varies With pH as a Continuum, Not a Threshold, Across the North China Plain.Frontiers in microbiology · 2022Article
- Short-term application of chicken manure under different nitrogen rates alters structure and co-occurrence pattern but not diversity of soil microbial community in wheat field.Frontiers in microbiology · 2022Article
- Space Is More Important than Season when Shaping Soil Microbial Communities at a Large Spatial Scale.mSystems · 2020Article
- CNS Infections Caused by Brown-Black Fungi.Journal of fungi (Basel, Switzerland) · 2019Review
- The preparation and clinical application of diagnostic DNA microarray for the detection of pathogens in intracranial bacterial and fungal infections.Experimental and therapeutic medicine · 2018Article
- Development of a Multilocus Sequence Typing System for Medically Relevant Bipolaris Species.Journal of clinical microbiology · 2015Article
- Extensive Exserohilum infection in a burn patient.JAAD case reports · 2015Article
- Utility of real-time PCR for detection of Exserohilum rostratum in body and tissue fluids during the multistate outbreak of fungal meningitis and other infections.Journal of clinical microbiology · 2015Article
- Outbreak of fungal infections associated with contaminated methylprednisolone acetate: an update.Current infectious disease reports · 2015Article
- Black Molds and Melanized Yeasts Pathogenic to Humans.Cold Spring Harbor perspectives in medicine · 2014Review
- Fungi associated with drug recalls and rare disease outbreaks.Journal of industrial microbiology & biotechnology · 2014Review
- Whole-genome analysis of Exserohilum rostratum from an outbreak of fungal meningitis and other infections.Journal of clinical microbiology · 2014Article
- Not over yet: fungal infections following methyl prednisolone injections smoulder on.Journal of clinical microbiology · 2014Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exserohilum rostratum was the major cause of an outbreak of fungal infections linked to injections of contaminated methylprednisolone acetate. Because almost 14,000 persons were exposed to product that was possibly contaminated with multiple fungal pathogens, there was unprecedented need for a rapid throughput diagnostic test that could detect both E. rostratum and other unusual agents of fungal infection. Here we report development of a novel PCR test that allowed for rapid and specific detection of fungal DNA in cerebrospinal fluid (CSF), other body fluids and tissues of infected individuals. The test relied on direct purification of free-circulating fungal DNA from fluids and subsequent PCR amplification and sequencing. Using this method, we detected Exserohilum rostratum DNA in 123 samples from 114 case-patients (28% of 413 case-patients for whom 627 samples were available), and Cladosporium DNA in one sample from one case-patient. PCR with novel Exserohilum-specific ITS-2 region primers detected 25 case-patients with samples that were negative using broad-range ITS primers. Compared to fungal culture, this molecular test was more sensitive: of 139 case-patients with an identical specimen tested by culture and PCR, E. rostratum was recovered in culture from 19 (14%), but detected by PCR in 41 (29%), showing a diagnostic sensitivity of 29% for PCR compared to 14% for culture in this patient group. The ability to rapidly confirm the etiologic role of E. rostratum in these infections provided an important contribution in the public health response to this outbreak.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.