ReviewProteomics2013
High-throughput cloning and expression library creation for functional proteomics.
Review in Proteomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Combining CRISPR-Cas-mediated terminal resolution with a novel genetic workflow to achieve high-diversity adenoviral libraries.Molecular therapy. Methods & clinical development · 2024Article
- FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins inFrontiers in bioengineering and biotechnology · 2022Article
- Article
- Construction of gateway-compatible baculovirus expression vectors for high-throughput protein expression and in vivo microcrystal screening.Scientific reports · 2020Article
- Recombinant β-Carotene Production byFrontiers in bioengineering and biotechnology · 2020Article
- Characterization of a Pyrethroid-DegradingFrontiers in microbiology · 2018Article
- TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression.PloS one · 2017Article
- Sequence homolog-based molecular engineering for shifting the enzymatic pH optimum.Synthetic and systems biotechnology · 2016Article
- High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives.Open biology · 2016Review
- Immunoproteomic Profiling of Antiviral Antibodies in New-Onset Type 1 Diabetes Using Protein Arrays.Diabetes · 2016Article
- Antiviral antibody profiling by high-density protein arrays.Proteomics · 2015Article
- Site-specific recombinational cloning using gateway and in-fusion cloning schemes.Current protocols in molecular biology · 2015Article
- Homologous recombinatorial cloning without the creation of single-stranded ends: exonuclease and ligation-independent cloning (ELIC).Molecular biotechnology · 2015Article
- Proteins interacting with cloning scars: a source of false positive protein-protein interactions.Scientific reports · 2015Article
- Expression analysis of genes and pathways associated with liver metastases of the uveal melanoma.BMC medical genetics · 2014Article
- Exploration of panviral proteome: high-throughput cloning and functional implications in virus-host interactions.Theranostics · 2014Article
- DNASU plasmid and PSI:Biology-Materials repositories: resources to accelerate biological research.Nucleic acids research · 2014Article
- Edgotype: a fundamental link between genotype and phenotype.Current opinion in genetics & development · 2013Review
- Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The study of protein function usually requires the use of a cloned version of the gene for protein expression and functional assays. This strategy is particularly important when the information available regarding function is limited. The functional characterization of the thousands of newly identified proteins revealed by genomics requires faster methods than traditional single-gene experiments, creating the need for fast, flexible, and reliable cloning systems. These collections of ORF clones can be coupled with high-throughput proteomics platforms, such as protein microarrays and cell-based assays, to answer biological questions. In this tutorial, we provide the background for DNA cloning, discuss the major high-throughput cloning systems (Gateway® Technology, Flexi® Vector Systems, and Creator(TM) DNA Cloning System) and compare them side-by-side. We also report an example of high-throughput cloning study and its application in functional proteomics. This tutorial is part of the International Proteomics Tutorial Programme (IPTP12).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.