ArticleJournal of microbiology (Seoul, Korea)2013
Isolation and characterization of a mycovirus in Lentinula edodes.
Article in Journal of microbiology (Seoul, Korea), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Deep sequencing of single Pleurotus ostreatus strain revealed mixed infection of seven RNA viruses.BMC genomics · 2026Article
- High-Throughput Sequencing-Guided Development and Validation of an RT-dPCR Assay for Sensitive Detection of Lentinula edodes Spherical Virus in Shiitake Mushrooms.Current microbiology · 2026Article
- Mycoviruses against plant pathogenic fungi: current understanding and future avenues of their potential implication.Archives of microbiology · 2026Review
- Rapid and visual detection of Lentinula edodes spherical virus using CRISPR/Cas12a-based RT-RPA.Virus genes · 2025Article
- New insights into temperature-impacted mycovirus-fungus interactions regulated by a microRNA inJournal of virology · 2025Article
- Characterization of a betaendornavirus isolated from the edible fungus Morchella sextelata.Archives of virology · 2025Article
- Changes in intra-host mycovirus population diversity after vertical and horizontal transmission.Virus evolution · 2025Article
- Transmission of Oyster Mushroom Spherical Virus to Progeny via Basidiospores and Horizontally to a New HostInternational journal of molecular sciences · 2024Article
- Horizontal and Vertical Transmission of a Mycovirus Closely Related to the Partitivirus RhsV717 That Confers Hypovirulence inViruses · 2023Article
- An Overview of Mycoviral Curing Strategies Used in Evaluating Fungal Host Fitness.Molecular biotechnology · 2023Review
- Oyster Mushroom Spherical Virus Crosses the Species Barrier and Is Pathogenic to a New HostInternational journal of molecular sciences · 2023Article
- Multiplex Detection ofCurrent issues in molecular biology · 2022Article
- A novel fungal negative-stranded RNA virus related to mymonaviruses in Auricularia heimuer.Archives of virology · 2022Article
- Diversity of mycoviruses in edible fungi.Virus genes · 2022Review
- Viruses infecting macrofungi.Virusdisease · 2018Review
- Viral Effects of a dsRNA Mycovirus (PoV-ASI2792) on the Vegetative Growth of the Edible MushroomMycobiology · 2016Article
- Genome sequence of a novel mycovirus of Rhizoctonia solani, a plant pathogenic fungus.Virus genes · 2015Article
- Characterization of a Novel Megabirnavirus from Sclerotinia sclerotiorum Reveals Horizontal Gene Transfer from Single-Stranded RNA Virus to Double-Stranded RNA Virus.Journal of virology · 2015Article
- Occurrence of dsRNA Mycovirus (LeV-FMRI0339) in the Edible Mushroom Lentinula edodes and Meiotic Stability of LeV-FMRI0339 among Monokaryotic Progeny.The plant pathology journal · 2013Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A mycovirus was isolated from an edible mushroom, Lentinula edodes, that was suffering from a severe epidemic. Fractionation of the diseased cell extract by isopycnic centrifugation with 50% CsCl revealed that the diseased mushroom was infected by Lentinula edodes spherical virus (LeSV), a new spherical virus with a diameter of 55 nm. The particle of LeSV encapsidated the 12 kb RNA genome by a 120 kDa coat protein. BLAST analysis of the partially sequenced LeSV genome showed 95% sequence identity with a putative RNA-dependent RNA polymerase (RdRp) gene of the mycovirus HKB, which was previously reported as being a double-stranded RNA (dsRNA) element. In contrast to HKB, the RNA genome in LeSV is encapsidated by the 120 kDa coat protein. To confirm that the LeSV coat protein is encoded by the viral genome, the N-terminal amino acid sequence of the coat protein was determined. The resulting N-terminal amino acid sequence, N-SALDVAPVVPELYFXXLEV-C, was found to be located in the middle of the HKB ORF1, suggesting that the LeSV coat protein was indeed encoded by the virus. To detect LeSV in L. edodes, a primer set targeting the RdRp gene was designed based on the partial sequence of the LeSV genome. RT-PCR analysis showed that 56 of the 84 commercially available dikaryotic cultivars carry LeSV. The transmission pattern of the virus was determined by analysing basidiospores from LeSV-infected and LeSV-free fruiting bodies. Nine out of 10 basidiospores from the LeSV-infected cultivars contained the virus while the spores from the LeSV-free parent were free of LeSV, suggesting that vertical transmission is the primary mode of LeSV propagation.
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