ArticleMolecules and cells2013
RNA-dependent RNA polymerase 6 is required for efficient hpRNA-induced gene silencing in plants.
Article in Molecules and cells, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- A novel ilarvirus protein CP-RT is expressed via stop codon readthrough and suppresses RDR6-dependent RNA silencing.PLoS pathogens · 2024Article
- Mutations in the WG and GW motifs of the three RNA silencing suppressors of grapevine fanleaf virus alter their systemic suppression ability and affect virus infectivity.Frontiers in microbiology · 2024Article
- Evidence of a novel silencing effect on transgenes in the Arabidopsis thaliana sperm cell.The Plant cell · 2023Article
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- Arabidopsis NF-YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation.International journal of molecular sciences · 2023Article
- ANGUSTIFOLIA negatively regulates resistance to Sclerotinia sclerotiorum via modulation of PTI and JA signalling pathways in Arabidopsis thaliana.Molecular plant pathology · 2022Article
- The proteome of developing barley anthers during meiotic prophase I.Journal of experimental botany · 2022Article
- A photoregulatory mechanism of the circadian clock in Arabidopsis.Nature plants · 2021Article
- The epigenetic factor FVE orchestrates cytoplasmic SGS3-DRB4-DCL4 activities to promote transgene silencing inScience advances · 2021Article
- Concerted increases ofPlant direct · 2021Article
- Identification of a consensus DNA-binding site for the TCP domain transcription factor TCP2 and its important roles in the growth and development of Arabidopsis.Molecular biology reports · 2021Article
- Induction of resistance to sugarcane mosaic virus by RNA interference targeting coat protein gene silencing in transgenic sugarcane.Molecular biology reports · 2021Article
- Calcium/Calmodulin-Mediated Defense Signaling: What Is Looming on the Horizon for AtSR1/CAMTA3-Mediated Signaling in Plant Immunity.Frontiers in plant science · 2021Review
- Role of the RNA-directed DNA Methylation pathway in the regulation of maternal effects inmicroPublication biology · 2021Article
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- Suppression ofViruses · 2019Article
- EBP1 nuclear accumulation negatively feeds back on FERONIA-mediated RALF1 signaling.PLoS biology · 2018Article
- A Single-Nucleotide Polymorphism in the Promoter of a Hairpin RNA Contributes toThe Plant cell · 2018Article
- TCP2 positively regulates HY5/HYH and photomorphogenesis in Arabidopsis.Journal of experimental botany · 2016Article
- Bensulfuron-Methyl Treatment of Soil Affects the Infestation of Whitefly, Aphid, andFrontiers in plant science · 2016Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, transgenes with inverted repeats are used to induce efficient RNA silencing, which is also frequently induced by highly transcribed sense transgenes. RNA silencing induced by sense transgenes is dependent on RNA-dependent RNA polymerase 6 (RDR6), which converts single-stranded (ss) RNA into double-stranded (ds) RNA. By contrast, it has been proposed that RNA silencing induced by self-complementary hairpin RNA (hpRNA) does not require RDR6, because the hpRNA can directly fold back on itself to form dsRNA. However, it is unclear whether RDR6 plays a role in hpRNA-induced RNA silencing by amplifying dsRNA to spread RNA silencing within the plant. To address the efficiency of hpRNA-induced RNA silencing in the presence or absence of RDR6, Wild type (WT, Col-0) and rdr6-11 Arabidopsis thaliana lines expressing green fluorescent protein (GFP) were generated and transformed with a GFP-RNA interference (RNAi) construct. Whereas most GFP-RNAi-transformed WT lines exhibited almost complete silencing of GFP expression in the T1 generation, various levels of GFP expression remained among the GFP-RNAi-transformed rdr6-11 lines. Homozygous expression of GFP-RNAi in the T3 generation was not sufficient to induce complete GFP silencing in several rdr6-11 lines. Our results indicate that RDR6 is required for efficient hpRNA-induced RNA silencing in plants.
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