ArticleCurrent genetics2013
Role of glutathione in the oxidative stress response in the fungal pathogen Candida glabrata.
Article in Current genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 40 citations in OpenAlex.
- The dual role of sertraline: From antidepressant to antifungal drug with broad metabolic disruption.Genetics and molecular biology · 2026Article
- Proteomic insight into the ectomycorrhizal fungus Laccaria bicolor illuminates the central role of glutathione-mediated resistance to cadmium toxicity.Mycorrhiza · 2025Article
- Glutathione-mediated redox regulation in Cryptococcus neoformans impacts virulence.Nature microbiology · 2024Article
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- Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog,International journal of inflammation · 2024Article
- The metabolites from traditional Chinese medicine targeting ferroptosis for cancer therapy.Frontiers in pharmacology · 2024Review
- Essential Role ofJournal of fungi (Basel, Switzerland) · 2023Article
- The Role of the Glutathione System in Stress Adaptation, Morphogenesis and Virulence of Pathogenic Fungi.International journal of molecular sciences · 2022Review
- Abf1 Is an Essential Protein That Participates in Cell Cycle Progression and Subtelomeric Silencing inJournal of fungi (Basel, Switzerland) · 2021Article
- Deletion of the Stress Response GeneJournal of fungi (Basel, Switzerland) · 2021Article
- Analysis of Volatile Molecules Present in the Secretome of the Fungal PathogenMolecules (Basel, Switzerland) · 2021Article
- The critical role ofApplied and environmental microbiology · 2021Article
- Tolerance to oxidative stress is associated with both oxidative stress response and inherent growth in a fungal wheat pathogen.Genetics · 2021Article
- Article
- The Transcription Factor Sfp1 Regulates the Oxidative Stress Response inMicroorganisms · 2019Article
- Review
- Physiological roles of bacillithiol in intracellular metal processing.Current genetics · 2016Review
- Anti-Immune Strategies of Pathogenic Fungi.Frontiers in cellular and infection microbiology · 2016Review
- Glutathione metabolism in Candida albicans resistant strains to fluconazole and micafungin.PloS one · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candida glabrata, an opportunistic fungal pathogen, accounts for 18-26 % of all Candida systemic infections in the US. C. glabrata has a robust oxidative stress response (OSR) and in this work we characterized the role of glutathione (GSH), an essential tripeptide-like thiol-containing molecule required to keep the redox homeostasis and in the detoxification of metal ions. GSH is synthesized from glutamate, cysteine, and glycine by the sequential action of Gsh1 (γ-glutamyl-cysteine synthetase) and Gsh2 (glutathione synthetase) enzymes. We first screened for suppressor mutations that would allow growth in the absence of GSH1 (gsh1∆ background) and found a single point mutation in PRO2 (pro2-4), a gene that encodes a γ-glutamyl phosphate reductase and catalyzes the second step in the biosynthesis of proline. We demonstrate that GSH is important in the OSR since the gsh1∆ pro2-4 and gsh2∆ mutant strains are more sensitive to oxidative stress generated by H2O2 and menadione. GSH is also required for Cadmium tolerance. In the absence of Gsh1 and Gsh2, cells show decreased viability in stationary phase. Furthermore, C. glabrata does not contain Saccharomyces cerevisiae high affinity GSH transporter ortholog, ScOpt1/Hgt1, however, our genetic and biochemical experiments show that the gsh1∆ pro2-4 and gsh2∆ mutant strains are able to incorporate GSH from the medium. Finally, GSH and thioredoxin, which is a second redox system in the cell, are not essential for the catalase-independent adaptation response to H2O2.
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