ArticleJournal of immunology (Baltimore, Md. : 1950)2013
Orai1 function is essential for T cell homing to lymph nodes.
Article in Journal of immunology (Baltimore, Md. : 1950), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Neutrophil Mechanosignaling Promotes Integrin Engagement With Endothelial Cells and Motility Within Inflamed Vessels.Frontiers in immunology · 2018Trial
- CRAC channels as targets for immunotherapy.Current opinion in immunology · 2026Review
- 7-Ketocholesterol promotes T cell migration through CaFrontiers in immunology · 2026Article
- Synthetic Biology Meets CaCells · 2024Review
- The role of ion channels in T cell function and disease.Frontiers in immunology · 2023Review
- How the Potassium Channel Response of T Lymphocytes to the Tumor Microenvironment Shapes Antitumor Immunity.Cancers · 2022Review
- Piezo1 channels restrain regulatory T cells but are dispensable for effector CD4Science advances · 2021Article
- CRAC Channels and Calcium Signaling in T Cell-Mediated Immunity.Trends in immunology · 2020Review
- Green Tea Polyphenol-Sensitive Calcium Signaling in Immune T Cell Function.Frontiers in nutrition · 2020Review
- Intramembrane ionic protein-lipid interaction regulates integrin structure and function.PLoS biology · 2018Article
- Intermittent CaeLife · 2017Article
- T-Time: A data repository of T cell and calcium release-activated calcium channel activation imagery.BMC research notes · 2017Article
- C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers.Experimental and therapeutic medicine · 2017Article
- Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Ca2+ signaling in cytoskeletal reorganization, cell migration, and cancer metastasis.BioMed research international · 2015Review
- Ion channels in innate and adaptive immunity.Annual review of immunology · 2015Review
- Interference with Ca(2+) release activated Ca(2+) (CRAC) channel function delays T-cell arrest in vivo.European journal of immunology · 2013Article
- Ca(2+) influx in T cells: how many ca(2+) channels?Frontiers in immunology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In T lymphocytes, Ca(2+) release-activated Ca(2+) (CRAC) channels composed of Orai1 subunits trigger Ag-induced gene expression and cell proliferation through the NFAT pathway. We evaluated the requirement of CRAC channel function for lymphocyte homing using expression of a dominant-negative Orai1-E106A mutant to suppress Ca(2+) signaling. To investigate homing and motility of human lymphocytes in immunocompromised mouse hosts, we transferred human lymphocytes either acutely or after stable engraftment after a second transfer from the same blood donor. Human and mouse lymphocyte homing was assessed, and cells were tracked within lymph nodes (LNs) by two-photon microscopy. Our results demonstrate that human T and B lymphocytes home into and migrate within the LNs of immunocompromised NOD.SCID mice similar to murine lymphocytes. Human T and B cells colocalized in atrophied or reconstituted mouse LNs, where T cells migrated in a random walk at velocities of 9-13 μm/min and B cells at 6 μm/min. Expression of Orai1-E106A inhibited CRAC channel function in human and mouse T cells, and prevented homing from high endothelial venules into murine LNs. Ca(2+) signals induced by CCL21 were also inhibited in T cells expressing Orai1-E106A. With CRAC channels inhibited, the high-affinity form of LFA-1 failed to become active, and T cells failed to migrate across endothelial cells in a transwell model. These results establish a requirement for CRAC channel-mediated Ca(2+) influx for T cell homing to LNs mediated by high-affinity integrin activation and chemokine-induced transendothelial migration.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.