ArticleThe EMBO journal2013
Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.
Article in The EMBO journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 54 citations in OpenAlex.
- Molecular anatomy of PLK1 master docking motifs.Nature communications · 2026Article
- Fat traffic control: S-acylation in axonal transport.Molecular pharmacology · 2025Review
- VezA/vezatin facilitates proper assembly of the dynactin complex in vivo.Cell reports · 2024Article
- Self-priming of Plk1 binding to BubR1 ensures accurate mitotic progression.Communications biology · 2024Article
- VezA/vezatin facilitates proper assembly of the dynactin complex in vivo.bioRxiv : the preprint server for biology · 2024Article
- Genetic enhancers of partial PLK1 inhibition reveal hypersensitivity to kinetochore perturbations.PLoS genetics · 2023Article
- Recent Progress on the Localization of PLK1 to the Kinetochore and Its Role in Mitosis.International journal of molecular sciences · 2022Review
- The Role of Mitotic Kinases and the RZZ Complex in Kinetochore-Microtubule Attachments: Doing the Right Link.Frontiers in cell and developmental biology · 2022Review
- Cryo-EM reveals the complex architecture of dynactin's shoulder region and pointed end.The EMBO journal · 2021Article
- Cell-cycle phospho-regulation of the kinetochore.Current genetics · 2021Review
- BUB1 and CENP-U, Primed by CDK1, Are the Main PLK1 Kinetochore Receptors in Mitosis.Molecular cell · 2021Article
- Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA.PLoS genetics · 2020Article
- Conformational diversity of dynactin sidearm and domain organization of its subunit p150.Molecular biology of the cell · 2020Article
- Mitotic kinase anchoring proteins: the navigators of cell division.Cell cycle (Georgetown, Tex.) · 2020Review
- Cargo-Mediated Activation of Cytoplasmic DyneinFrontiers in cell and developmental biology · 2020Review
- p25 of the dynactin complex plays a dual role in cargo binding and dynactin regulation.The Journal of biological chemistry · 2018Article
- The actin capping protein in Aspergillus nidulans enhances dynein function without significantly affecting Arp1 filament assembly.Scientific reports · 2018Article
- LIM kinase1 regulates mitotic centrosome integrity via its activity on dynein light intermediate chains.Open biology · 2018Article
- Dynarrestin, a Novel Inhibitor of Cytoplasmic Dynein.Cell chemical biology · 2018Article
- Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis.Scientific reports · 2017Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Dynactin is a protein complex required for the in vivo function of cytoplasmic dynein, a microtubule (MT)-based motor. Dynactin binds both dynein and MTs via its p150(Glued) subunit, but little is known about the 'pointed-end complex' that includes the protein subunits Arp11, p62 and the p27/p25 heterodimer. Here, we show that the p27/p25 heterodimer undergoes mitotic phosphorylation by cyclin-dependent kinase 1 (Cdk1) at a single site, p27 Thr186, to generate an anchoring site for polo-like kinase 1 (Plk1) at kinetochores. Removal of p27/p25 from dynactin results in reduced levels of Plk1 and its phosphorylated substrates at kinetochores in prometaphase, which correlates with aberrant kinetochore-MT interactions, improper chromosome alignment and abbreviated mitosis. To investigate the structural implications of p27 phosphorylation, we determined the structure of human p27. This revealed an unusual left-handed β-helix domain, with the phosphorylation site located within a disordered, C-terminal segment. We conclude that dynactin plays a previously undescribed regulatory role in the spindle assembly checkpoint by recruiting Plk1 to kinetochores and facilitating phosphorylation of important downstream targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.