ArticlePloS one2013
Blood and dried blood spot telomere length measurement by qPCR: assay considerations.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Telomere length analysis from minimally-invasively collected samples: Methods development and meta-analysis of the validity of different sampling techniques: American Journal of Human Biology.American journal of human biology : the official journal of the Human Biology Council · 2021Pooled it
- CumulativeFrontiers in cellular and infection microbiology · 2026Article
- Relationship between telomere length and postoperative delirium: a single center prospective observational pilot study.Scientific reports · 2025Observational
- How sample handling distorts telomere studies.Scientific reports · 2025Article
- Cross-tissue comparison of telomere length and quality metrics of DNA among individuals aged 8 to 70 years.PloS one · 2024Article
- Preserving avian blood and DNA sampled in the wild: A survey of personal experiences.Ecology and evolution · 2022Article
- The prevalence of nurse burnout and its association with telomere length pre and during the COVID-19 pandemic.PloS one · 2022Article
- Longitudinal Follow-Up of Blood Telomere Length in HIV-Exposed Uninfected Children Having Received One Year of Lopinavir/Ritonavir or Lamivudine as Prophylaxis.Children (Basel, Switzerland) · 2021Article
- Telomere dynamics across the early life course: Findings from a longitudinal study in children.Psychoneuroendocrinology · 2021Article
- Article
- Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.PloS one · 2021Article
- Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature.PloS one · 2021Article
- An Optimised Step-by-Step Protocol for Measuring Relative Telomere Length.Methods and protocols · 2020Article
- Telomere Biology and Human Phenotype.Cells · 2019Review
- Telomere length measurement by qPCR - Summary of critical factors and recommendations for assay design.Psychoneuroendocrinology · 2019Review
- Effect of master mixes on the measurement of telomere length by qPCR.Molecular biology reports · 2018Article
- Dried Blood Spots for Global Health Diagnostics and Surveillance: Opportunities and Challenges.The American journal of tropical medicine and hygiene · 2018Review
- Premature Spinal Bone Loss in Women Living with HIV is Associated with Shorter Leukocyte Telomere Length.International journal of environmental research and public health · 2018Article
- Validation of quantitative polymerase chain reaction with Southern blot method for telomere length analysis.Future science OA · 2018Article
- Blood Mitochondrial DNA Content in HIV-Exposed Uninfected Children with Autism Spectrum Disorder.Viruses · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Measurement of telomere length is crucial for the study of telomere maintenance and its role in molecular pathophysiology of diseases and in aging. Several methods are used to measure telomere length, the choice of which usually depends on the type and size of sample to be assayed, as well as cost and throughput considerations. The goal of this study was to investigate the factors that may influence the reliability of qPCR-based relative telomere length measurements in whole blood. Day to day intra-individual variability, types of blood anticoagulant, sample storage conditions, processing and site of blood draw were investigated. Two qPCR-based methods to measure telomere length (monoplex vs. multiplex) were also investigated and showed a strong correlation between them. Freezing and thawing of the blood and storage of the blood at 4°C for up to 4 days did not affect telomere length values. Telomere lengths in dried blood spots were significantly higher than both whole blood and peripheral mononuclear blood cells, and were highly correlated with both. We found that telomere length measurements were significantly higher in dried blood spots collected directly from fingertip prick compared to dried blood spots prepared with anticoagulated whole blood collected from the finger, and non-blotted whole blood taken from both finger and arm venipuncture. This suggests that DNA from cells blotted on paper is not equivalent to that collected from venipuncture whole blood, and caution should be taken when comparing between blood sample types.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.