ArticlePloS one2013
New insights from the oyster Crassostrea rhizophorae on bivalve circulating hemocytes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 117 citations in OpenAlex.
- Development of long-term oyster explant cultures enable sustained tissue-specific activity and reveal novel cellular behaviours.Biochemistry and biophysics reports · 2026Article
- Article
- Morphological and functional characterization of the oysterFish and shellfish immunology reports · 2023Article
- Immune diversity in lophotrochozoans, with a focus on recognition and effector systems.Computational and structural biotechnology journal · 2023Review
- Bivalve Haemocyte Subpopulations: A Review.Frontiers in immunology · 2022Review
- Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oysterFrontiers in immunology · 2022Article
- Time-Course of the Innate Immune Response of the Terrestrial CrustaceanFrontiers in immunology · 2022Article
- Crayfish hemocytes develop along the granular cell lineage.Scientific reports · 2021Article
- Stressor-Dependant Changes in Immune Parameters in the Terrestrial Isopod Crustacean,Nanomaterials (Basel, Switzerland) · 2021Article
- Long Term Exposure to Virgin and Recycled LDPE Microplastics Induced Minor Effects in the Freshwater and Terrestrial CrustaceansPolymers · 2021Article
- Stem Cells and Innate Immunity in Aquatic Invertebrates: Bridging Two Seemingly Disparate Disciplines for New Discoveries in Biology.Frontiers in immunology · 2021Review
- Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific OysterFrontiers in immunology · 2020Article
- Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate,Frontiers in immunology · 2020Article
- Functional and evolutionary perspectives on gill structures of an obligate air-breathing, aquatic snail.PeerJ · 2019Article
- Microbial Diseases of Bivalve Mollusks: Infections, Immunology and Antimicrobial Defense.Marine drugs · 2017Review
- Article
- Article
- The crustacean central nervous system in focus: subacute neurodegeneration induces a specific innate immune response.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hemocytes are the first line of defense of the immune system in invertebrates, but despite their important role and enormous potential for the study of gene-environment relationships, research has been impeded by a lack of consensus on their classification. Here we used flow cytometry combined with histological procedures, histochemical reactions and transmission electron microscopy to characterize the hemocytes from the oyster Crassostrea rhizophorae. Transmission electron microscopy revealed remarkable morphological characteristics, such as the presence of membranous cisternae in all mature cells, regardless of size and granulation. Some granular cells contained many cytoplasmic granules that communicated with each other through a network of channels, a feature never previously described for hemocytes. The positive reactions for esterase and acid phosphatase also indicated the presence of mature cells of all sizes and granule contents. Flow cytometry revealed a clear separation in complexity between agranular and granular populations, which could not be differentiated by size, with cells ranging from 2.5 to 25 µm. Based on this evidence we suggest that, at least in C. rhizophorae, the different subpopulations of hemocytes may in reality be different stages of one type of cell, which accumulates granules and loses complexity (with no reduction in size) as it degranulates in the event of an environmental challenge.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.