ArticleStudies in mycology2013
Heterogeneity in liquid shaken cultures of Aspergillus niger inoculated with melanised conidia or conidia of pigmentation mutants.
Article in Studies in mycology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- The α-(1,3)-glucan synthase gene agsE impacts the secretome of Aspergillus niger.Antonie van Leeuwenhoek · 2023Article
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- Ionic silver functionalized ovine forestomach matrix - a non-cytotoxic antimicrobial biomaterial for tissue regeneration applications.Biomaterials research · 2019Article
- Functional distinction of hyphal compartments.Scientific reports · 2017Article
- Cell Biology of Hyphal Growth.Microbiology spectrum · 2017Review
- Comparative genomics and transcriptome analysis of Aspergillus niger and metabolic engineering for citrate production.Scientific reports · 2017Article
- Fungal Spores Viability on the International Space Station.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2016Article
- Shedding light on Aspergillus niger volatile exometabolome.Scientific reports · 2016Article
- Switching from a unicellular to multicellular organization in an Aspergillus niger hypha.mBio · 2015Article
- Sorting of Streptomyces cell pellets using a complex object parametric analyzer and sorter.Journal of visualized experiments : JoVE · 2014Article
- Monitoring the metabolic state of fungal hyphae and the presence of melanin by nonlinear spectral imaging.Applied and environmental microbiology · 2013Article
- Development in Aspergillus.Studies in mycology · 2013Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Black pigmented conidia of Aspergillus niger give rise to micro-colonies when incubated in liquid shaken medium. These micro-colonies are heterogeneous with respect to gene expression and size. We here studied the biophysical properties of the conidia of a control strain and of strains in which the fwnA, olvA or brnA gene is inactivated. These strains form fawn-, olive-, and brown-coloured conidia, respectively. The ΔolvA strain produced larger conidia (3.8 μm) when compared to the other strains (3.2-3.3 μm). Moreover, the conidia of the ΔolvA strain were highly hydrophilic, whereas those of the other strains were hydrophobic. The zeta potential of the ΔolvA conidia in medium was also more negative when compared to the control strain. This was accompanied by the near absence of a rodlet layer of hydrophobins. Using the Complex Object Parametric Analyzer and Sorter it was shown that the ratio of individual hyphae and micro-colonies in liquid shaken cultures of the deletion strains was lower when compared to the control strain. The average size of the micro-colonies of the control strain was also smaller (628 μm) than that of the deletion strains (790-858 μm). The size distribution of the micro-colonies of the ΔfwnA strain was normally distributed, while that of the other strains could be explained by assuming a population of small and a population of large micro-colonies. In the last set of experiments it was shown that relative expression levels of gpdA, and AmyR and XlnR regulated genes correlate in individual hyphae at the periphery of micro-colonies. This indicates the existence of transcriptionally and translationally highly active and lowly active hyphae as was previously shown in macro-colonies. However, the existence of distinct populations of hyphae with high and low transcriptional and translational activity seems to be less robust when compared to macro-colonies grown on solid medium.
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