ArticleMolecular biology of the cell2013
An analogue-sensitive approach identifies basal body rotation and flagellum attachment zone elongation as key functions of PLK in Trypanosoma brucei.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- Identification of feeding apparatus components in a heterotrophic marine flagellate.Journal of cell science · 2026Article
- Chemical genetics reveals Leishmania KKT2 and CRK9 kinase activity is required for cell cycle progression.PLoS pathogens · 2026Article
- Revealing spatio-temporal dynamics with long-term trypanosomatid live-cell imaging.PLoS pathogens · 2022Article
- Drug Target Validation of the Protein KinaseMicrobiology spectrum · 2021Article
- The kinetoplastid-specific phosphatase KPP1 attenuates PLK activity to facilitate flagellum inheritance inScience signaling · 2021Article
- Alternate histories of cytokinesis: lessons from the trypanosomatids.Molecular biology of the cell · 2020Review
- Polo-like kinase in trypanosomes: an odd member out of the Polo family.Open biology · 2020Article
- Rapid block of pre-mRNA splicing by chemical inhibition of analog-sensitive CRK9 in Trypanosoma brucei.Molecular microbiology · 2020Article
- Regulated protein stabilization underpins the functional interplay among basal body components inThe Journal of biological chemistry · 2020Article
- Who Needs a Contractile Actomyosin Ring? The Plethora of Alternative Ways to Divide a Protozoan Parasite.Frontiers in cellular and infection microbiology · 2019Review
- Identification of TOEFAZ1-interacting proteins reveals key regulators of Trypanosoma brucei cytokinesis.Molecular microbiology · 2018Article
- TbSmee1 regulates hook complex morphology and the rate of flagellar pocket uptake in Trypanosoma brucei.Molecular microbiology · 2018Article
- Let's get fISSical: fast in silico synchronization as a new tool for cell division cycle analysis.Parasitology · 2018Review
- A functional analysis of TOEFAZ1 uncovers protein domains essential for cytokinesis inJournal of cell science · 2017Article
- Article
- A novel protein kinase is essential in bloodstream Trypanosoma brucei.International journal for parasitology · 2016Article
- Divergent polo box domains underpin the unique kinetoplastid kinetochore.Open biology · 2016Article
- Review
- The Centriole Cartwheel Protein SAS-6 in Trypanosoma brucei Is Required for Probasal Body Biogenesis and Flagellum Assembly.Eukaryotic cell · 2015Article
- Proteomic identification of novel cytoskeletal proteins associated with TbPLK, an essential regulator of cell morphogenesis in Trypanosoma brucei.Molecular biology of the cell · 2015Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Polo-like kinases are important regulators of cell division, playing diverse roles in mitosis and cytoskeletal inheritance. In the parasite Trypanosoma brucei, the single PLK homologue TbPLK is necessary for the assembly of a series of essential organelles that position and adhere the flagellum to the cell surface. Previous work relied on RNA interference or inhibitors of undefined specificity to inhibit TbPLK, both of which have significant experimental limitations. Here we use an analogue-sensitive approach to selectively and acutely inhibit TbPLK. T. brucei cells expressing only analogue-sensitive TbPLK (TbPLK(as)) grow normally, but upon treatment with inhibitor develop defects in flagellar attachment and cytokinesis. TbPLK cannot migrate effectively when inhibited and remains trapped in the posterior of the cell throughout the cell cycle. Using synchronized cells, we show that active TbPLK is a direct requirement for the assembly and extension of the flagellum attachment zone, which adheres the flagellum to the cell surface, and for the rotation of the duplicated basal bodies, which positions the new flagellum so that it can extend without impinging on the old flagellum. This approach should be applicable to the many kinases found in the T. brucei genome that lack an ascribed function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.