Evidence map›Paper›PMID 23447634›Full record

ArticleJournal of clinical microbiology2013

Comparative evaluation of conventional and real-time PCR assays for detecting Bacteroides fragilis in clinical samples.

Joseph Papaparaskevas, Victoria Mela, Dimitra P Houhoula, Angeliki Pantazatou, Georgios L Petrikkos, Athanassios Tsakris

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Bloodstream infection caused byThe Journal of international medical research · 2021
    Article
  6. Article
  7. Simultaneous Supplementation ofFrontiers in microbiology · 2018
    Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Joseph PapaparaskevasDepartment of Microbiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece. ipapapar@med.uoa.gr
Victoria Mela
Dimitra P Houhoula
Angeliki Pantazatou
Georgios L Petrikkos
Athanassios Tsakris

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A conventional PCR and a real-time PCR for detecting Bacteroides fragilis were evaluated against clinical specimens. Analytical sensitivities were 100 and 40 fg of DNA, respectively. Detection limits were 100 and 10 CFU/ml, respectively. A total of six culture-negative specimens were positive by PCR. Altering the gold standard from "positive culture" to "positive culture or both PCR assays positive" resulted in sensitivities of 91.7% and 100%, respectively, and in specificities of 100% and 98.6%, respectively.

Indexed as

Real-Time Polymerase Chain ReactionBacteroides fragilisBacteroides InfectionsDNA, BacterialFecesHumansLimit of DetectionRNA, Ribosomal, 16SSensitivity and SpecificityDNA, BacterialRNA, Ribosomal, 16S

Identifiers

PMID23447634
PMCPMC3647933

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.