ArticlePlant physiology2013
Understanding the role of defective invertases in plants: tobacco Nin88 fails to degrade sucrose.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- Loss of the plastidial hexokinase CsHXK3 triggers metabolic starvation and ROS imbalance leading to pollen abortion in cucumber.The Plant journal : for cell and molecular biology · 2026Article
- Cell wall invertase improves grain nutrition via regulating sugar and hormone metabolism gene expression in transgenic soybean.Annals of botany · 2026Article
- Genome-wide characterization of invertases in Arabica coffee and its progenitors reveals putative genes involved in fruit development.Functional & integrative genomics · 2025Article
- Roles and Regulations of Acid Invertases in Plants: Current Knowledge and Future Perspectives.Plants (Basel, Switzerland) · 2025Review
- Insights into Sucrose Metabolism and Its Ethylene-Dependent Regulation in Cucumis melo L.Molecular biotechnology · 2025Review
- Article
- Genome-wide identification and analysis of the invertase gene family in tobacco (Frontiers in plant science · 2023Article
- Evidence for Light and Tissue Specific Regulation of Genes Involved in Fructan Metabolism inPlants (Basel, Switzerland) · 2022Article
- Diurnal Changes in Water Soluble Carbohydrate Components in Leaves and Sucrose AssociatedInternational journal of molecular sciences · 2020Article
- Integrating Sugar Metabolism With Transport: Elevation of Endogenous Cell Wall Invertase Activity Up-RegulatesFrontiers in genetics · 2020Article
- Article
- A 6&1-FEH Encodes an Enzyme for Fructan Degradation and Interact with Invertase Inhibitor Protein in Maize (International journal of molecular sciences · 2019Article
- Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink.Frontiers in plant science · 2019Article
- Physiological Traits for Shortening Crop Duration and Improving Productivity of Greengram (Frontiers in plant science · 2019Article
- Expression analysis of Cell wall invertase under abiotic stress conditions influencing specialized metabolism in Catharanthus roseus.Scientific reports · 2018Article
- Genome-Wide Survey of Invertase Encoding Genes and Functional Characterization of an Extracellular Fungal Pathogen-Responsive Invertase inInternational journal of molecular sciences · 2018Article
- Novel fructan exohydrolase: unique properties and applications for human health.Journal of experimental botany · 2018Article
- Aberrant seed development inHorticulture research · 2018Article
- Floral Metabolism of Sugars and Amino Acids: Implications for Pollinators' Preferences and Seed and Fruit Set.Plant physiology · 2017Review
- Chicory R2R3-MYB transcription factors CiMYB5 and CiMYB3 regulate fructan 1-exohydrolase expression in response to abiotic stress and hormonal cues.Journal of experimental botany · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell wall invertases (cwINVs), with a high affinity for the cell wall, are fundamental enzymes in the control of plant growth, development, and carbon partitioning. Most interestingly, defective cwINVs have been described in several plant species. Their highly attenuated sucrose (Suc)-hydrolyzing capacity is due to the absence of aspartate-239 (Asp-239) and tryptophan-47 (Trp-47) homologs, crucial players for stable binding in the active site and subsequent hydrolysis. However, so far, the precise roles of such defective cwINVs remain unclear. In this paper, we report on the functional characterization of tobacco (Nicotiana tabacum) Nin88, a presumed fully active cwINV playing a crucial role during pollen development. It is demonstrated here that Nin88, lacking both Asp-239 and Trp-47 homologs, has no invertase activity. This was further supported by modeling studies and site-directed mutagenesis experiments, introducing both Asp-239 and Trp-47 homologs, leading to an enzyme with a distinct Suc-hydrolyzing capacity. In vitro experiments suggest that the addition of Nin88 counteracts the unproductive and rather aspecific binding of tobacco cwINV1 to the wall, leading to higher activities in the presence of Suc and a more efficient interaction with its cell wall inhibitor. A working model is presented based on these findings, allowing speculation on the putative role of Nin88 in muro. The results presented in this work are an important first step toward unraveling the specific roles of plant defective cwINVs.
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