ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2013
Prolonged high fat/alcohol exposure increases TRPV4 and its functional responses in pancreatic stellate cells.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Metabolism and Targeted Therapy of Fibrosis in Chronic Pancreatitis: A Review.International journal of medical sciences · 2025Review
- Piezo1-mediated stellate cell activation causes pressure-induced pancreatic fibrosis in mice.JCI insight · 2022Article
- Pancreatic Stellate Cells and Metabolic Alteration: Physiology and Pathophysiology.Frontiers in physiology · 2022Review
- Role of the TRP Channels in Pancreatic Ductal Adenocarcinoma Development and Progression.Cells · 2021Review
- Signaling in the Physiology and Pathophysiology of Pancreatic Stellate Cells -Frontiers in physiology · 2020Review
- Article
- Involvement of Neural Transient Receptor Potential Channels in Peripheral Inflammation.Frontiers in immunology · 2020Review
- Ion Channels Orchestrate Pancreatic Ductal Adenocarcinoma Progression and Therapy.Frontiers in pharmacology · 2020Review
- Regulation of Pain and Itch by TRP Channels.Neuroscience bulletin · 2018Review
- Biology of pancreatic stellate cells-more than just pancreatic cancer.Pflugers Archiv : European journal of physiology · 2017Review
- Novel and Experimental Therapies in Chronic Pancreatitis.Digestive diseases and sciences · 2017Review
- Ion channels in control of pancreatic stellate cell migration.Oncotarget · 2017Article
- A Mouse Model of Chronic Pancreatitis Induced by an Alcohol and High Fat Diet.The open pain journal · 2017Article
- Alcohol and high fat induced chronic pancreatitis: TRPV4 antagonist reduces hypersensitivity.Neuroscience · 2015Article
- Effects of treadmill running and rutin on lipolytic signaling pathways and TRPV4 protein expression in the adipose tissue of diet-induced obese mice.Journal of physiology and biochemistry · 2015Article
- Transient receptor potential vanilloid 4 inhibits rat HSC-T6 apoptosis through induction of autophagy.Molecular and cellular biochemistry · 2015Article
- Pharmacological attenuation of chronic alcoholic pancreatitis induced hypersensitivity in rats.World journal of gastroenterology · 2015Article
- Role of pancreatic fat in the outcomes of pancreatitis.Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The present study investigated transient receptor potential vanilloid type 4 (TRPV4) ion channels in pancreatic stellate cells (PSCs) isolated from rats with high-fat and alcohol diet (HFA)-induced chronic pancreatitis. TRPV4 is a calcium-permeable nonselective ion channel responsive to osmotic changes, alcohol metabolites arachidonic acid, anandamide, their derivatives, and injury-related lipid mediators. Male Lewis rats were fed HFA for 6-8 wk before isolation and primary culture of PSCs. Control PSCs were harvested from rats fed standard chow. Immunoreactivity for cytoskeletal protein activation product α-smooth muscle actin (α-SMA) and platelet-derived growth factor receptor-β subunit (PDGFR-β) characterized the cells as PSCs. TRPV4 expression increased in PSCs of HFA-fed rats and control cultures after alcohol treatment (50 mM). Cell responses to activation of inducible TRPV4 were assessed with live cell calcium imaging. Threefold increased and sustained intracellular calcium mobilization responses occurred in 70% of pancreatic stellate cells from HFA-fed rats in response to TRPV4 activators arachidonic acid, lipid second messenger, phorbol ester 4 α-phorbol 12,13-didecanoate (4αPDD), and 50% hypoosmotic media compared with relatively unresponsive PSCs from control rats. Activation responses were attenuated by nonselective TRPV channel blocker ruthenium red. Tumor necrosis factor-α (TNF-α, 1 ng/ml, 16 h) increased responses to 4αPDD in control PSCs. These findings implicate TRPV4-mediated calcium responses inducible after HFA exposure and inflammation in reactive responses of activated PSCs that impair pancreatic function, such as responsiveness to cytokines and the deposition of collagen fibrosis that precipitates ductal blockage and pain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.