ArticleProceedings. Biological sciences2013
A biogenic amine and a neuropeptide act identically: tyramine signals through calcium in Drosophila tubule stellate cells.
Article in Proceedings. Biological sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Pathobiont and symbiont contribute to microbiota homeostasis through Malpighian tubules-gut countercurrent flow in Bactrocera dorsalis.The ISME journal · 2024Article
- Octopamine and tyramine signalling in Aedes aegypti: Molecular characterization and insight into potential physiological roles.PloS one · 2023Article
- Transcriptional and functional motifs defining renal function revealed by single-nucleus RNA sequencing.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Updates on ion and water transport by the Malpighian tubule.Current opinion in insect science · 2021Review
- A unique Malpighian tubule architecture inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Review
- Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications.International journal of molecular sciences · 2020Review
- The septate junction protein Mesh is required for epithelial morphogenesis, ion transport, and paracellular permeability in theAmerican journal of physiology. Cell physiology · 2020Article
- Physiology, Development, and Disease Modeling in theGenetics · 2020Review
- Specialized stellate cells offer a privileged route for rapid water flux inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- secCl is a cys-loop ion channel necessary for the chloride conductance that mediates hormone-induced fluid secretion in Drosophila.Scientific reports · 2019Article
- Identification of multiple functional receptors for tyramine on an insect secretory epithelium.Scientific reports · 2017Article
- In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin.Journal of visualized experiments : JoVE · 2016Article
- The Splice Isoforms of the Drosophila Ecdysis Triggering Hormone Receptor Have Developmentally Distinct Roles.Genetics · 2016Article
- Tracing the evolutionary origins of insect renal function.Nature communications · 2015Article
- Chloride channels in stellate cells are essential for uniquely high secretion rates in neuropeptide-stimulated Drosophila diuresis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Phylogenetic investigation of Peptide hormone and growth factor receptors in five dipteran genomes.Frontiers in endocrinology · 2013Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Insect osmoregulation is subject to highly sophisticated endocrine control. In Drosophila, both Drosophila kinin and tyramine act on the Malpighian (renal) tubule stellate cell to activate chloride shunt conductance, and so increase the fluid production rate. Drosophila kinin is known to act through intracellular calcium, but the mode of action of tyramine is not known. Here, we used a transgenically encoded GFP::apoaequorin translational fusion, targeted to either principal or stellate cells under GAL4/UAS control, to demonstrate that tyramine indeed acts to raise calcium in stellate, but not principal cells. Furthermore, the EC(50) tyramine concentration for half-maximal activation of the intracellular calcium signal is the same as that calculated from previously published data on tyramine-induced increase in chloride flux. In addition, tyramine signalling to calcium is markedly reduced in mutants of NorpA (a phospholipase C) and itpr, the inositol trisphosphate receptor gene, which we have previously shown to be necessary for Drosophila kinin signalling. Therefore, tyramine and Drosophila kinin signals converge on phospholipase C, and thence on intracellular calcium; and both act to increase chloride shunt conductance by signalling through itpr. To test this model, we co-applied tyramine and Drosophila kinin, and showed that the calcium signals were neither additive nor synergistic. The two signalling pathways thus represent parallel, independent mechanisms for distinct tissues (nervous and epithelial) to control the same aspect of renal function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.