ArticleAutophagy2013
Qualitative and quantitative characterization of protein-phosphoinositide interactions with liposome-based methods.
Article in Autophagy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed.
- CROP: a retromer-PROPPIN complex mediating membrane fission in the endo-lysosomal system.The EMBO journal · 2022Article
- Liposomes as Carriers of Membrane-Associated Proteins and Peptides for Mass Spectrometric Analysis.Angewandte Chemie (International ed. in English) · 2021Article
- Exploring Phosphoinositide Binding Using Native Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Determining the Lipid-Binding Specificity of SMP Domains: An ERMES Subunit as a Case Study.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Liposome Flotation Assay for Studying Interactions Between Rubella Virus Particles and Lipid Membranes.Bio-protocol · 2018Article
- The ARF guanine nucleotide exchange factor GBF1 is targeted to Golgi membranes through a PIP-binding domain.Journal of cell science · 2018Article
- Both Sphingomyelin and Cholesterol in the Host Cell Membrane Are Essential for Rubella Virus Entry.Journal of virology · 2018Article
- Quantitative Evaluation of Viral Protein Binding to Phosphoinositide Receptors and Pharmacological Inhibition.Analytical chemistry · 2017Article
- Direct interaction of the Golgi V-ATPase a-subunit isoform with PI(4)P drives localization of Golgi V-ATPases in yeast.Molecular biology of the cell · 2017Article
- An overview of macroautophagy in yeast.Journal of molecular biology · 2016Review
- Specificity of Collybistin-Phosphoinositide Interactions: IMPACT OF THE INDIVIDUAL PROTEIN DOMAINS.The Journal of biological chemistry · 2016Article
- Receptor-mediated Endocytosis 8 Utilizes an N-terminal Phosphoinositide-binding Motif to Regulate Endosomal Clathrin Dynamics.The Journal of biological chemistry · 2015Article
- Characterization of PROPPIN-Phosphoinositide Binding and Role of Loop 6CD in PROPPIN-Membrane Binding.Biophysical journal · 2015Article
- Biophysical methods for the characterization of PTEN/lipid bilayer interactions.Methods (San Diego, Calif.) · 2015Review
- Membrane targeting of the Spir·formin actin nucleator complex requires a sequential handshake of polar interactions.The Journal of biological chemistry · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We characterized phosphoinositide binding of the S. cerevisiae PROPPIN Hsv2 qualitatively with density flotation assays and quantitatively through isothermal titration calorimetry (ITC) measurements using liposomes. We discuss the design of these experiments and show with liposome flotation assays that Hsv2 binds with high specificity to both PtdIns3P and PtdIns(3,5)P 2. We propose liposome flotation assays as a more accurate alternative to the commonly used PIP strips for the characterization of phosphoinositide-binding specificities of proteins. We further quantitatively characterized PtdIns3P binding of Hsv2 with ITC measurements and determined a dissociation constant of 0.67 µM and a stoichiometry of 2:1 for PtdIns3P binding to Hsv2. PtdIns3P is crucial for the biogenesis of autophagosomes and their precursors. Besides the PROPPINs there are other PtdIns3P binding proteins with a link to autophagy, which includes the FYVE-domain containing proteins ZFYVE1/DFCP1 and WDFY3/ALFY and the PX-domain containing proteins Atg20 and Snx4/Atg24. The methods described could be useful tools for the characterization of these and other phosphoinositide-binding proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.