ArticleBiochemistry2013
Aging-associated enzyme human clock-1: substrate-mediated reduction of the diiron center for 5-demethoxyubiquinone hydroxylation.
Article in Biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Nematode-trapping fungus Arthrobotrys oligospora is hungry for iron-chelating agent COQ7 of nematodes.Natural products and bioprospecting · 2026Article
- In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis.Nature catalysis · 2024Article
- Mitochondrial Factors in the Cell Nucleus.International journal of molecular sciences · 2023Review
- Predicting and Understanding the Pathology of Single Nucleotide Variants in HumanAntioxidants (Basel, Switzerland) · 2022Article
- Structure and functionality of a multimeric human COQ7:COQ9 complex.Molecular cell · 2022Article
- Mössbauer-based molecular-level decomposition of the Saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei.Metallomics : integrated biometal science · 2022Article
- Manganese-driven CoQ deficiency.Nature communications · 2022Article
- Article
- An Isoprene Lipid-Binding Protein Promotes Eukaryotic Coenzyme Q Biosynthesis.Molecular cell · 2019Article
- Coenzyme QEssays in biochemistry · 2018Review
- Biochemistry of Mitochondrial Coenzyme Q Biosynthesis.Trends in biochemical sciences · 2017Review
- A single biochemical activity underlies the pleiotropy of the aging-related protein CLK-1.Scientific reports · 2017Article
- Plastoquinone and Ubiquinone in Plants: Biosynthesis, Physiological Function and Metabolic Engineering.Frontiers in plant science · 2016Review
- Differential Protein Distribution between the Nucleus and Mitochondria: Implications in Aging.Frontiers in genetics · 2016Review
- CLD1 Reverses the Ubiquinone Insufficiency of Mutant cat5/coq7 in a Saccharomyces cerevisiae Model System.PloS one · 2016Article
- Yeast Coq9 controls deamination of coenzyme Q intermediates that derive from para-aminobenzoic acid.Biochimica et biophysica acta · 2015Article
- Mitochondrial COQ9 is a lipid-binding protein that associates with COQ7 to enable coenzyme Q biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Electron transfer control in soluble methane monooxygenase.Journal of the American Chemical Society · 2014Article
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Authors and funding
4 authors.
Funding
Abstract
The mitochondrial membrane-bound enzyme Clock-1 (CLK-1) extends the average longevity of mice and Caenorhabditis elegans, as demonstrated for Δclk-1 constructs for both organisms. Such an apparent impact on aging and the presence of a carboxylate-bridged diiron center in the enzyme inspired this work. We expressed a soluble human CLK-1 (hCLK-1) fusion protein with an N-terminal immunoglobulin binding domain of protein G (GB1). Inclusion of the solubility tag allowed for thorough characterization of the carboxylate-bridged diiron active site of the resulting GB1-hCLK-1 by spectroscopic and kinetic methods. Both UV-visible and Mössbauer experiments provide unambiguous evidence that GB1-hCLK-1 functions as a 5-demethoxyubiquinone-hydroxylase, utilizing its carboxylate-bridged diiron center. The binding of DMQn (n = 0 or 2) to GB1-hCLK-1 mediates reduction of the diiron center by nicotinamide adenine dinucleotide (NADH) and initiates O2 activation for subsequent DMQ hydroxylation. Deployment of DMQ to mediate reduction of the diiron center in GB1-hCLK-1 improves substrate specificity and diminishes consumption of NADH that is uncoupled from substrate oxidation. Both Vmax and kcat/KM for DMQ hydroxylation increase when DMQ0 is replaced by DMQ2 as the substrate, which demonstrates that an isoprenoid side chain enhances enzymatic hydroxylation and improves catalytic efficiency.
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